C1q对巨噬细胞抗结核中作用的影响及其机制研究

Study on effect of C1q on anti-TB action of macrophages and its mechanism

目的探讨C1q在肺结核患者中的表达及其对巨噬细胞抗结核作用的机制。方法选择活动性肺结核、结核性胸膜炎、结核杆菌潜伏感染者、健康对照者各30例,于入院次日或健康体检时抽取外周静脉血10~20mL。对有胸腔积液者抽取胸腔积液50mL,对行手术治疗的患者取肺组织标本1cm×1cm×1cm。采用实时荧光定量PCR(RT-PCR)检测各组外周血巨噬细胞中C1q表达水平,采用免疫组化染色检测肺组织内巨噬细胞C1q表达水平。结果活动性肺结核组、结核性胸膜炎组患者外周血C1qA的表达水平明显高于健康对照组及潜伏感染组(P<0.05)。结核性胸膜炎组患者胸腔积液C1qA的表达水平高于活动性肺结核组(P<0.05),且两组患者胸腔积液中C1q的表达水平均高于其在外周血中的表达(P<0.05)。活动性肺结核组及结核性胸膜炎组巨噬细胞胞浆中C1q均呈强表达,在多核巨噬细胞中C1q的表达较弱。结论结核病患者外周血C1q表达增高,病变局部的免疫反应可能更为明显。C1q在结核巨噬细胞中强表达,可能与巨噬细胞分化和表型有相关性。

Objective To investigate the C1q expression in the patients with pulmonary tuberculosis (TB) and its mechanism on the anti-TB role of macrophages .Methods Each 30 cases of active pulmonary TB ,tuberculous pleurisy ,latent Mycobacterium tu-berculosis infection and healthy controls were selected .Peripheral venous blood 10-20 mL was collected on the next day of admis-sion or on the time for physical examination .Pleural fluid 50 mL was extracted in the patients with hydrothorax and the lung tissue specimens 1 cm × 1 cm × 1 cm was taken in the operative patients .The real-time quantitative PCR(RT-PCR) was adopted to detect the express C1q in peripheral macrophages and the C1q expressing in macrophages of the lung tissue was detected by immunohisto-chemical staining .Results The peripheral blood C1qA expression level in the active pulmonary TB group and the tuberculous pleu-risy group was significantly higher than that in the healthy control group and the latent TB infection group (P<0 .05) .The C1qA expression level in hydrothorax in the tuberculous pleurisy group was higher than that in the active pulmonary TB group (P<0 .05) ,and the C1q expression in hydrothorax in these two groups was higher than that in peripheral blood (P<0 .05) .C1q was strongly expressed in macrophage cytoplasma in the active pulmonary TB group and the TB pleurisy group ,but weakly expressed in the multinuclear macrophages .Conclusion The peripheral blood C1qA expression level in the active pulmonary TB group and the tuberculous pleurisy group was significantly higher than that in the healthy control group and the latent TB infection group (P<0 .05) .The C1qA expression level in hydrothorax in the tuberculous pleurisy group was higher than that in the active pulmonary TB group (P<0 .05) ,and the C1q expression in hydrothorax in these two groups was higher than that in peripheral blood (P<0 .05) . C1q was strongly expressed in macrophage cytoplasma in the active pulmonary TB group and the TB pleurisy group ,but weakly ex-pressed in the multinuclear macrophages .