Bmi-1基因表达与浸润性乳腺导管癌临床病理特征的关系

Relation between Bmi-1 gene expression and clinicopathological characteristics in breast infiltrating ductal carcinoma

目的研究浸润性乳腺导管癌及癌旁组织中Bmi-1基因表达水平及差异,及其与浸润性乳腺导管癌临床病理特征的关系。方法应用实时荧光定量PCR检测82例浸润性乳腺导管癌患者手术切除癌组织及相应癌旁组织中的Bmi-1mRNA,比较两者间表达的差异;研究基因表达水平与年龄、肿瘤大小、TNM分期、组织分化程度、淋巴结转移等临床病理特征的关系和相关性。结果 Bmi-1mRNA在浸润性乳腺导管癌及癌旁组织中均可检出,癌组织中的基因表达水平显著高于癌旁组织(P=0.001)。Bmi-1基因表达水平与年龄无关,与肿瘤大小、肿瘤组织分化程度、有无淋巴结转移相关。结论 Bmi-1基因的高表达与浸润性乳腺导管癌的侵袭和发展相关,其检测结果可作为浸润性乳腺导管癌早期诊断及预后的分子水平参考指标。

Objective To investigate the Bmi-1 gene expression level and difference in breast infiltrating ductal carcinoma and pericarcinomatous tissue and its relation with the clinicopathological characteristics of breast infiltrating ductal carcinoma .Methods The tissue specimens were obtained from the resected carcinoma tissue and corresponding pericarcinomatous tissues in 82 patients with breast infiltrating ductal carcinoma and Bmi-1 mRNA was detected by using the real-time quantitative reverse transcriptional PCR (qRT-PCR) .The expression differences between them were compared ;the relation and the correlation between the gene ex-pression with the clinicopathological characteristics of the age ,tumor size ,TNM staging ,histodifferentiation degree and lymphatic metstasis were studied .Results Bmi-1 mRNA could be detected in breast infiltrating ductal carcinoma and pericarcinomatous tis-sue ,and the gene expression level in the carcinoma tissue was significantly higher than that in the pericarcinomatous tissues (P=0 .001) .The expression level of Bmi-1 gene had no relation with the age and was correlated with the tumor size ,tumor′s differentia-tion degree and lymphatic metastasis .Conclusion The high expression of Bmi-1 gene is correlated with the invasion and progress of breast infiltrating ductal carcinoma ,its detection results could be used as the reference index in the molecular level for the early di-agnosis of breast infiltrating ductal carcinoma and prognosis .