不孕相关抗体检测女性不孕症的实验研究

Experimental study on infertility related antibody detection in female infertility

目的探讨6种自身免疫抗体与女性不孕症间是否存在相关性。方法采用蛋白微阵列技术联合检测140例不孕症患者血清中抗精子抗体(ASAb)、抗子宫内膜抗体(AEmAb)、抗卵巢抗体(AVoAb)、抗透明带抗体(AZpAb)、抗滋养层抗体(ATAb)和抗人绒毛膜促性腺激素抗体(AhCG Ab),并评价这6种抗体与女性不孕症的相关性。结果与对照组相比,不孕组中除AVoAb外,其他5种抗体检测的阳性率比较差异有统计学意义(P<0.05)。不孕组中,6种抗体联合检测的阳性率与单独检测比较差异有统计学意义(P<0.05)。原发不孕组和继发不孕组中6种自身抗体的阳性率与对照组比较差异有统计学意义(P<0.05);不同病因引起的继发不孕中6种自身抗体的检测阳性率间比较差异无统计学意义(P>0.05)。不孕组中ANA的阳性率与对照组比较,差异无统计学意义(P>0.05),但实验者ANA抗体滴度多大于或等于1∶100(6/7),核型多为核颗粒型(5/7),与对照组中阳性滴度较低不同。不孕相关性抗体阳性患者和抗体阴性患者中ANA阳性率比较差异有统计学意义(P<0.05)。结论ASAb、AZpAb、AEmAb、AhCGAb和ATAb与女性不孕症存在相关性,6种自身免疫抗体的联合检测可明显提高实验的灵敏度。

Objective To study whether the correlation exists between 6 kinds of autoimmune antibodies and female infertility . Methods The protein microarray technique was adopted to detect serum antibodies of anti-sperm antibodies(ASAb) ,anti-endome-trial(AEmAb) ,anti-zona pellucida(AZpAb) ,anti-ovarian(AVoAb) ,anti-hCG(AhCG Ab) and anti-trophoblastic(ATAb) in 140 ca-ses of female infertility and the correlation between these 6 kinds of antibody with female infertility was evaluated .Results Com-pared with the control group ,except AvoAb ,the positive rates of other 5 kinds of antibody had statistically significant differences (P<0 .05) .In the infertility group ,the positive rate of the combined detection of these 6 kinds of autoimmune antibody had statisti-cal difference compared with that of single item detection (P<0 .05) .The positive rate of these 6 kinds of autoimmune antibody in the primary infertility group and the secondary infertility group had statistical difference compared with the control group ;the posi-tive rate of these 6 kinds of autoimmune antibody in the secondary infertility caused by different etiologies had no statistical differ-ence (P>0 .05) .The positive rate of ANA had no statistical difference between the infertility group and the control group (P>0 .05) ,but the titers of ANA in the experimenter were ≥1∶100(6/7) and most of karyotypes were nuclear coarse (5/7) ,which was differed from the low positive titer in the control group .The positive rate of ANA had statistical difference between the infertility related antibody positive and the infertility related antibody negative in the infertility patients .Conclusion ASAb ,AZpAb AEmAb , AhCGAb and ATAb are related with female sterility .The combined detection of these 6 kinds of antibody can significantly increase the detection sensitivity .