全自动血凝分析仪不同方法检测血浆纤维蛋白原的对比研究

Comparative study on different methods for detecting plasma fibrinogen by automated coagulation analyzers

目的:对比分析全自动血凝分析仪PT衍生法与Clauss法测定血浆纤维蛋白原结果,探讨其准确性及临床价值。方法选择2013年3~5月期间在该院接受诊治的156例患者血样本,25份正常质控品,13例D-二聚体阳性标本使用PT衍生法与Clauss法测定法分析。结果 Clauss法与PT衍生物法在Sysmex CA-1500全自动血凝分析仪上测定Fib 25次重复率良好;当Fib<1.0g/L时,两种方法检测结果差异有统计学意义(P<0.05);当Fib在1.0~2.0g/L、2.1~3.0g/L、3.1~4.0g/L,两种方法检测结果差异无统计学意义(P>0.05);当Fib在4.1~5.0g/L时,两种方法检测结果差异有统计学意义(P<0.05);当Fib>5.0g/L时,两种方法检测结果差异有统计学意义(P<0.05);D-二聚体阳性标本13例使用PT衍生法测定结果显著高于Clauss法,差异有统计学意义(t=8.530,P<0.05)。结论测定血浆纤维蛋白原两种方法PT衍生法与Clauss法存在差异,但Clauss法测定准确性高于特异性高,临床测定血浆纤维蛋白应以Clauss法首选。

Objective To comparatively analyze the results of the PT derivative method and the Clauss method for detecting fi-brinogen(Fib) by the automated coagulation analyzer and to explore their accuracy and clinical value .Methods Fib was detected in 156 blood samples selected from the patients in this hospital from March to May 2013 ,25 normal control samples and 13 positive samples of D-dimer (DD) by the TP derivative method and the Clauss method .Results The Clauss method and the PT derivative method by Sysmex CA-1500 coagulation analyzer had good repetitive rate in measuring Fib for 25 times ;when Fib<1 .0 g/L ,the results detected by these two methods had statistically significant difference (P<0 .05);when Fib in the range of 1 .0 -2 .0 g/L , 2 .1-3 .0 g/L ,3 .1-4 .0 g/L ,the detection results had no statistically significant difference between the two methods (P>0 .05);when Fib in the range of 4 .1 -5 .0 g/L ,the detection results had statistically significant difference (P<0 .05);when Fib>5 .0 g/L ,the detection results had statistically significant difference (P<0 .05);in 13 DD positive samples ,the detection results of the PT derivative method were significantly higher than those of the Clauss method with statistically significant difference (t=8 .530 , P<0 .05) .Conclusion The difference exists between the PT derivative method and the Clauss method for detecting Fib ,but the ac-curacy of the Clauss method is higher than its specificity ,the Clauss method should be the first choice for the clinical determination of plasma Fib .