血液病患者信号转导及转录激活子5和叉头状转录因子3的表达

Expressions of STAT5 and Foxp3 in patients with hematological disease

目的观察恶性血液病患者外周血中单个核细胞磷酸化STAT5及Foxp3的表达水平及其相关性。方法采用蛋白磷酸化流式细胞术分别检测45例急性白血病患者(AL)、23例慢性白血病(CL)、29例骨髓增生异常综合征(MDS)、20例重型再生障碍性贫血(SAA)及38例对照组外周血单个核细胞磷酸化STAT5(P-STAT5)及Foxp3阳性细胞的百分率。结果PSTAT5表达AL组(2.29±0.79)%、CL组(2.45±0.88)%、MDS组(2.21±0.75)%都较对照组(0.54±0.15)%增高,差异具有统计学意义(P<0.05),SAA组P-STAT5表达(0.43±0.17)%较对照组降低,差异无统计学意义(P>0.05);Foxp3表达AL组(3.86±1.13)%、CL组(3.60±1.10)%、MDS组(4.01±1.19)%高于对照组(0.89±0.38)%,差异具有统计学意义(P<0.05),SAA组Foxp3表达(0.57±0.21)%较对照组降低,差异具有统计学意义(P<0.05)。AL、CL、MDS、SAA各组P-STAT5与Foxp3表达水平都呈正相关(P<0.05)。结论 STAT5及Foxp3可能共同参与了AL、CL、MDS、SAA这几类恶性血液病的发生过程,同时测定可以提供有临床意义的实验依据。

Objective To observe the expressions and its correlation of P‐STAT5 and Foxp3 in peripheral blood mononuclear cells of patients with hematologic malignancies .Methods The percentage of P‐STAT5 and Foxp3 positive cells in the peripheral blood were tested in 45 cases of patients with acute leukemia (AL) ,23 cases of chronic leukemia(CL) ,29 cases of myelodysplastic syndrome(MDS) ,20 cases of severe aplastic anemia(SAA) and 38 controls by using phospho‐flow cytometry .Results The per‐centage of P‐STAT5 of AL patients group (2 .29 ± 0 .79)% ,CL(2 .45 ± 0 .88)% and MDS(2 .21 ± 0 .75)% were higher than con‐trol group(0 .54 ± 0 .15)% ,statistically significant difference (P< 0 .05);The percentage of P‐STAT5 of SAA group (0 .43 ± 0 .17)% reduced than the control group ,no statistically significant difference (P> 0 .05) .The percentage of Foxp3 of patients group with AL (3 .86 ± 1 .13)% ,CL (3 .60 ± 1 .10)% ,MDS (4 .01 ± 1 .19)% were higher than that of control group (0 .89 ± 0 .38)% ,statistically significant difference (P<0 .05);The percentage of Foxp3 of SAA group(0 .57 ± 0 .21)% reduced than the control group ,difference has statistically significant (P<0 .05) .The expression level of P‐STAT5 and Foxp3 in AL ,CL ,MDS and SAA were respectively positive correlation (P<0 .05) .Conclusion STAT5 and Foxp3 may jointly participate in the process of the occurrence of AL ,CL ,MDS and SAA ,detect them can provide experimental basis for clinical significance at the same time detec‐tion .