东莞汉族人PCSK9基因SNP与冠心病及其预后关系探讨

Dongguan Han patients with coronary artery PCSK9 gene SNP and its prognosis

目的探讨东莞汉族人PCSK9基因E670G位点SNP与冠心病(CAD)及其预后的关系。方法选择在该院CAD患者100例及非CAD患者100例为研究对象,取患者血液,提取DNA并采用PCR法分析PCSK9基因E670G位点SNP,并采用基因测序法验证。采用酶法检测患者血脂水平,并随访CAD患者采用他汀类治疗后血清脂质水平的变化、心血管事件发生率。结果冠心病(CAD)组血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平显著高于健康对照组,HDL-C显著低于健康对照组,差异具有统计学意义(P<0.05)。基因型主要为AA即298bp和152bp的纯合子,其次为AG即450bp和298bp、152bp的杂合子,尚未检测到450bp GG纯合子基因型,等位基因频率分布符合Hardy-Weinberg平衡。CAD组患者AA基因型的患者LDL-C水平显著低于AG基因型,HDL-C水平显著高于AG基因型的患者(P<0.05)。随访半年发生心血管的例数总共为27例,AA基因型占66.7%,AG基因型占33.3%,G等位基因携带者心血管事件例数和平均病变支数差异具有统计学意义(P<0.05)。结论 PCSK9基因E670G多态性与LDL-C、HDL-C水平及CAD病变程度相关,CAD患者中G等位基因的携带可能增加发病概率和再发病风险。

Objective To investigate the gene E670G SNP loci with coronary heart disease and its relationship Dongguan Han PCSK9 prognosis .Methods In our hospital 100 patients with coronary heart disease and 100 cases of non‐coronary heart disease patients for the study ,patients taking blood ,DNA was extracted and analyzed gene PCSK9 E670G SNP locus by PCR ,using gene sequencing validation .Lipid levels in patients using enzymatic detection and follow‐up of patients with coronary heart disease chan‐ges in serum lipid levels after statin therapy ,the incidence of cardiovascular events .Results CAD group TC ,LDL‐C levels were sig‐nificantly higher than the healthy control group ,HDL‐C was significantly lower than the healthy control group ,the difference was statistically significant (P<0 .05) .AA genotype that was mainly 298 bp and 152 bp of homozygotes ,followed by AG that was 450 bp and 298 bp ,152 bp heterozygotes ,had not been detected 450 bp GG homozygous genotype ,allele frequency distributions in Har‐dy‐Weinberg equilibrium .LDL‐C levels in patients with CAD patients was significantly lower than AA genotype AG genotype , HDL‐C levels were significantly higher in patients with AG genotype (P<0 .05) .Number of cardiovascular patients were followed up six months totaled 27 cases ,AA genotype accounted for 66 .7% ,AG genotype accounted for 33 .3% ,Gallele and the average number of cases of cardiovascular disease events count a statistically significant difference (P<0 .05) .Conclusion PCSK9 E670G polymorphism and LDL‐C ,HDL‐C levels and CAD severity gene‐related ,CAD patients carrying G allele may increase the risk of disease and the risk of again .