生化试剂对循环酶法测定血清总胆汁酸的干扰原因

Biochemical reagents for circulating enzymatic determination of serum total bile acid interference

目的探讨生化试剂对循环酶法测定血清总胆汁酸(TBA)的干扰原因,探寻消除干扰的办法。方法以门冬氨酸氨基转移酶、碱性磷酸酶、钙等20个生化常规项目试剂作用作为观察对象,确认对TBA具有显著干扰的生化试剂,且经过设定特殊程序减小仪器的携带污染。结果测定混合血清当中20份TBA水平,其平均值为(8.16±0.012)μmol/L;在20个观察项目当中,尿酸试剂(UA)对于测定总胆汁酸(TBA)结果影响差异具有统计学意义(P<0.05),存在系统误差;总胆固醇(CHOL)、三酰甘油(TG)项目试剂对于测定TBA结果影响差异具有统计学意义(P<0.05),对于TBA测定具有明显干扰。结论全自动化生化分析仪测定TBA时存在项目之间的互相污染,因此设定有效、合理的特殊冲洗程序能够有效地对消灭携带污染对TBA测定干扰。

Objective To study the interference reasonsof biochemical reagents for serum Total Bile Acid assay kit by enzyme cycle method ,explore the way to eliminate interference .Methods The AST ,ALP ,Ca etc .20 biochemical conventional reagents as research object ,confirming those biochemical reagents of significant interference to total bile acid assay kit by enzyme cycle method , and reducing the carry pollution of instrument by setting special procedures .Results The determination of 20 copies of serum total bile acid content in mix serum ,their average was (8 .16 ± 0 .012)μmol/L ;Among 20 observation projects ,UA reagents for the de‐termination of total bile acid result influence had statistically significant (P<0 .05) ,there was system error ;CHOL ,TG project rea‐gents had statistically significant(P<0 .05) ,and had obvious interferences .Conclusion There are the pollution between each other in the determination of Total Bile Acid project by Automatic Biochemical Analyzer ,so the effective ,reasonable and special wash pro‐gram by seting can effectively eliminate the carrying pollution to determinate the interference of Total Bile Aid .