1种血红蛋白电泳液标本改良方法的探讨

Introduce a improved method for the production of hemoglobin liquid by use of the hemoglobin electrophoresis alkaline

目的探讨1种血红蛋白电泳液改良制备方法的运用。方法对一批EDTA抗凝全血标本先用加样器吸取血液中沉降的红细胞,滴入到生理盐水中,借助吸液嘴从液面自上至下反复冲打,使红细胞均匀地悬浮在液体当中,上机离心,使红细胞充分沉淀在底部,倾倒出离心后的上清液,以去除血浆及杂质,留余量的红细胞,加蒸馏水或溶血素,溶解红细胞,双手端起试管架利用手腕轻轻前后摇动数次至溶血液澄清透明。结果该文方法与传统方法作比较,将二者结果再与国际地贫协会推荐的方法高效液湘色谱法(HPLC)测得的结果比较,3种初筛方法再与地贫基因确认方法比较结果符合性,从而可以客观地评价试验的可靠性。结论该方法 SOP操作所泳电泳图效果一致、省时省力、节省试剂成本、而且整个制备血红蛋白液的过程中不需接触任何化学试剂,不会对环境有污染,减小了有毒试剂对人体的伤害。

Objective To introduce a improved method for the production of hemoglobin liquid by use of the hemoglobin electro‐phoresis alkaline .Methods First ,we used the pipette to absorb the settlement of red blood cells from a batch of EDTA anticoagula‐ted whole blood specimens ,then dropped them into the 0 .9% saline washed human erythrocytes .With the help of the pipette nozzle we pipet from the liquid surface to bottom repeatedly ,made the red cells suspended in the liquid evenly .The samples should be cen‐trifuged and the red blood cells fully deposited at the bottom ,then poured the supernatant after centrifugation to remove the impuri‐ties in plasma and leave the allowance of red blood cells .We add distilled water or hemolysin to lysis RBC .Hands up test tubes rack using wrist gently back and forth several times until the hemolysis was clear and transparent .Results Compared the results of the modified method and the traditional method ,then the two results compared with the results of HPLC recommended by international association of thalassemia method .Compared the results of three screening methods with that of the thalassemia gene identification method .So we could objectively evaluate the reliability of the test .Conclusion The result of the improved method are same to the electrophoretogram by SOP operation ,it will be more efficient and reduce the cost of reagent .The whole process of preparing hemo‐globin solution doesn′t contact with any chemical reagent .So there will be no pollution to the environment ,and it also reduces the harmful of toxic reagent to the human body .