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诱导性多能干细胞技术用于研究人类疾病 被引量：1

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摘要 获得遗传学上与患者相配的大量细胞是再生医学的梦想。诱导性多能干细胞技术通过在培养皿中培养细胞来研究发病机理,建立疾病模型,观察疾病的分子机制,并发现新细胞基础的药物。诱导多能干(iPS)细胞从非多能细胞诱导产生,但拥有相似于胚胎干(ES)细胞的多能性,Takahashi等[1-4]首先实现了这个突破,在体外成功表达4个转录因子,即Oct4、Sox2、Klf4和c-Myc,这个步骤不需要卵母细胞。
作者 阮光萍 刘菊芬 姚翔 何洁 王金祥 杨建勇 庞荣清 潘兴华
机构地区 成都军区昆明总医院干细胞工程实验室
出处 《国际检验医学杂志》 CAS 2015年第12期1753-1756,共4页 International Journal of Laboratory Medicine
基金 国家自然科学基金项目(31172170) 国家重点基础研究发展计划项目(973计划)(2012CB518106) 云南省高新技术产业发展(201204) 国家支撑计划资助项目(2014BAI01B01)
关键词 诱导性多能干细胞 重编程 体细胞 人类疾病
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献17

  • 1Matthias Stadtfeld,Effie Apostolou,Hidenori Akutsu,Atsushi Fukuda,Patricia Follett,Sridaran Natesan,Tomohiro Kono,Toshi Shioda,Konrad Hochedlinger.Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature . 2010
  • 2Michael J. Boland,Jennifer L. Hazen,Kristopher L. Nazor,Alberto R. Rodriguez,Wesley Gifford,Greg Martin,Sergey Kupriyanov,Kristin K. Baldwin.Adult mice generated from induced pluripotent stem cells. Nature . 2009
  • 3Zhao Xiao-yang,Li Wei,Lv Zhuo,Liu Lei,Tong Man,Hai Tang,Hao Jie,Guo Chang-long,Ma Qing-wen,Wang Liu,Zeng Fanyi,Zhou Qi.iPS cells produce viable mice through tetraploid complementation. Nature . 2009
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二级参考文献10

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  • 7Nakagawa M, Koyanagi M, Tanabe K, et al. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechno12008; 26:101-106.
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共引文献11

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国际检验医学杂志
2015年 第12期

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