苏州市学龄前儿童末梢血铜、铁、锌、钙、镁五元素参考范围调查

The reference range of copper,iron,zinc,calcium and magnesium in peripheral blood of preschool children in Suzhou

目的根据ISO15189《医学实验室质量和能力的专用要求》,建立苏州市学龄前儿童末梢血铜、铁、锌、钙、镁五元素的参考范围。方法使用原子吸收光谱法检测2014年4~5月间苏州市区幼儿园健康体检儿童3 992例末梢血铜、铁、锌、钙、镁浓度,根据年龄分为3~<4岁组1 511例,男750例,女761例;4~<5岁组1 169例,男570例,女599例;5~<6岁组1 312例,男604例,女708例。按照NCCLS发布的临床实验室如何确定和建立生物参考区间C28-A2文件要求剔除离群值后进行参考范围的计算。结果铜、铁、锌、钙、镁五元素结果在不同性别及年龄组间无统计学差异,合并各年龄及性别组进行计算。苏州市学龄前儿童末梢血五元素参考范围为:铜:11.47~32.53μmol/L,铁:6.86~9.80mmol/L,锌:64.46~133.02μmol/L,钙:1.47~2.09mmol/L,镁:1.21~1.75mmol/L。结论儿童末梢血铜、铁、锌、钙、镁含量存在地区差异,每个地区应该建立符合本地区儿童的参考范围。

Objective Accodring to the guideline of Reference Intervals in the Clinical Laboratory(ISO15189) ,to establish the reference range of Cu ,Fe ,Zn ,Ca and Mg in the peripheral blood of preschool children in Suzhou .Methods Using atomic absorp-tion spectrometry to detect the levels of Cu ,Fe ,Zn ,Ca and Mg in peripheral blood of 3 992 Kindergarden children in Suzhou from Apr to May 2014 ,according the age ,the children were divided into three groups ,1 511 cases in 3 to 4 years old group including 750 boys ,761 girls ,1 169 cases in 4 to 5 years old group including 570 boys ,599 girls ,1 312 cases in 5 to 6 years old group inclu-ding 604 boys 708 girls .According to NCCLS''s guideline C28 -A2 to define the reference range .Results The concentration of copper ,iron ,zinc ,calcium ,magnesium had no significant differences in different gender and age groups(P>0 .05) ,the reference ranges in peripheral blood of the preschool children in Suzhou were Cu 11 .47-32 .53 umol/L ,Fe 6 .86-9 .80 mmol/L ,Zn 64 .46-133 .02 umol/L ,Ca 1 .47-2 .09 mmol/L ,Mg 1 .21 -1 .75 mmol/L .Conclusion There are regional difference on the levels of Cu ,Fe ,Zn ,Ca ,Mg in children''s peripheral blood ,each region should to establish the local reference range follow the guideline .