IM患儿外周血单核细胞TLR2、TLR9及CD19^+、CD23^+表达水平及意义

The expression of TLR2,TLR9,CD19^+ and CD23^+ in peripheral blood mononuclear cells in children with infectious mononucleosis

目的探究传染性单核细胞增多症(IM)患儿外周血单核细胞TLR2、TLR9及CD19^+、CD23^+表达水平及意义。方法选取2017年1月至2018年1月该院儿科初治的IM患儿40例作为研究对象,根据IM治疗过程分期分为急性期组、恢复期组,同时选取同期体检健康40例儿童作为对照组,对所有参与该研究儿童外周血单核细胞的TLR2mRNA、TLR9mRNA的表达水平,以及外周淋巴B细胞CD19^+和CD19^+CD23^+的阳性表达率进行测定。对比分析各组以上指标的变化情况。结果急性期组CD19^+和CD19^+CD23^+阳性表达率显著低于恢复期组和对照组,差异有统计学意义(P<0.05);恢复期组CD19^+和CD19^+CD23^+阳性表达率显著低于对照组,差异有统计学意义(P<0.05);急性期组TLR2mRNA和TLR9mRNA表达水平显著高于恢复期组和对照组,差异有统计学意义(P<0.05);恢复期组TLR2mRNA和TLR9mRNA表达水平显著高于对照组,差异有统计学意义(P<0.05)。结论 IM患儿的外周血单核细胞TLR2、TLR9及CD19^+、CD23^+在不同时期的阳性表达率存在差异,这些指标可能参与了IM发病全过程。

Objective To explore the expression and significance of peripheral blood mononuclear cells TLR2,TLR9,CD19^+and CD23^+ in children with infectious mononucleosis(IM).Methods 40 children with IM who were first treated in the department of pediatrics from January 2017 to January 2018 were selected as study subjects.According to the stages of IM treatment,they were divided into acute stage group and convalescent stage group.At the same time,40 healthy children were selected as control group.The expressions of TLR2 and TLR9 in peripheral blood mononuclear cells,CD19^+ and CD19^+CD23^+ in peripheral lymphocyte of all the children involved in the study were detected.The expression rate was determined.The changes of above indicators in each group were compared and analyzed.Results The positive expression rates of CD19^+ and CD19^+CD23^+ in the acute phase group were significantly lower than those in the convalescent phase group and the control group(P<0.05).The positive expression rates of CD19^+ and CD19^+CD23^+ in the convalescent phase group were significantly lower than those in the control group(P<0.05).The expression levels of TLR2 and TLR9 in the acute phase group were significantly higher than those in the convalescent phase group and the control group(P<0.05).The expression levels of TLR2 and TLR9 in convalescent group were significantly higher than those in control group(P<0.05).Conclusion The expression levels of TLR2,TLR9,and CD19^+ and CD23^+ in peripheral blood mononuclear cells in children with IM are different at different periods,and these indicators may be involved in the whole process of IM.