外周血miR-15a、miR-16检测在献血者早期乙型肝炎病毒感染筛查中意义

Significance of peripheral blood miR-15a,miR-16 measurement in screening for early hepatitis B virus infection in blood donors

目的探讨微小RNA(miRNA)miR-15a、miR-16在献血者乙型肝炎病毒(HBV)早期感染筛查中的意义。方法选择2017年2月至2018年1月该中心血站的无偿献血血液样本156份,其中有49份血液样本常规酶联免疫吸附测定(ELISA)双试剂筛查HBsAg呈阳性,而核酸HBV检测呈阴性,作为E单阳组;54份血液样本经ELISA筛查和核酸检测(NAT)均为阳性,作为EN双阳组;其余63份血液样本经ELISA筛查和NAT均为阴性,作为正常组。采用实时荧光定量PCR检测3组献血者miR-15a、miR-16的表达情况。结果相较于正常组样本,E单阳组和EN双阳组血液样本miR-15a和miR-16表达均明显下调(P<0.05);且EN双阳组血液样本miR-15a和miR-16表达下调较E单阳组更为明显(P<0.05)。经Pearson相关性分析,miR-15a和miR-16与EN双阳组患者HBV DNA拷贝数呈负相关(r=-0.725、-0.866,P<0.05)。结论 miRNA-15a、miRNA-16表达下调与HBV早期感染和复制活动密切相关,在HBV感染的早期筛查中具有潜在的应用价值。

Objective To investigate the significance of microRNA(miRNA)miRNA-15 aand miRNA-16 in screening for early hepatitis B virus(HBV)infection in blood donors.Methods Totally 156 blood samples were collected from the center blood station from February 2017 to January 2018.Among them,49 blood samples were positive for HBsAg screened by routine double-reagents enzyme-linked immunosorbent assay(ELISA),which was inconsistent with the results of nucleic acid test for HBV,and were set as single-positive E group.Totally 54 blood samples were both positive in ELISA and nucleic acid test(NAT)which were set as double-positive EN group.The remaining 63 blood samples were both negative by ELISA and NAT,which were set as normal group.Real-time fluorescent quantitative PCR was used to detect the expression of miRNA-15 aand miRNA-16 in the three groups of blood samples.Results The relative expressions of miR-15 aand miR-16 in single-positive E group and double-positive EN group were lower than the normal group(P<0.05).The relative expressions of miR-15 aand miR-16 in double-positive EN group were lower than the single-positive E group(P<0.05).According to the result of Pearson analysis,the relative expressions of both miR-15 aand miR-16 were negatively correlated with the copy number of HBV DNA in double-positive EN group group(r=-0.725,-0.866,P<0.05).Conclusion The miR-15 aand miR-16 can be used for the earlystage diagnosis of HBV infection,which well be recommended as the potential biomarkers for screening the donors.