南德温杂交肉牛生长分化因子10基因多态性与体尺性状的关联分析

GDF10 Gene Polymorphism and its Association with Body Measurement Traits in South Devon Hybrid Progeny

以98头南德温杂交肉牛血样为材料,随机抽样构建混合DNA池,利用高分辨率熔解曲线分析技术和直接测序法检测生长分化因子10(growth differentiation factor 10,GDF10)基因的序列变异,研究分析GDF10基因的多态性。采用SHEsis和PHASE软件对GDF10基因多态位点进行配对连锁不平衡和单倍型分析,采用SPSS 17.0进行基因多态位点和单倍型组合与体尺性状关联性分析。结果显示,南德温杂交肉牛GDF10基因检测到3个多态位点1113(G/A)、9569(G/A)和9628(G/A);关联分析表明,南德温杂交肉牛GDF10基因不同突变位点的基因型与体斜长、体高、胸围和管围差异显著(P<0.05);单倍型分析后在群体中发现8种单倍型组合,其中AAA、GGA与南德温杂交肉牛的体高、体斜长、胸围和管围显著相关(P<0.05)。由此推断,南德温杂交肉牛GDF10基因3个多态位点和2个单倍型组合与体斜长、体高、胸围和管围显著相关,可以作为肉牛生产性状的候选分子标记,为肉牛遗传资源开发与利用提供科学依据。

Blood samples were randomly selected from genomic DNA of 98 South Devon hybrid progeny to construct DNA pool,and sequence variations and polymorphism of growth differentiation factor 10(GDF10)gene were analyzed by high resolution melting curve(HRM)analysis techniques and direct sequencing.SHEsis and PHASE were used to screen the SNPs in GDF10 gene by the wave height of sequencing map.The association between genotypes and body measurement was evaluated by SPSS 17.0.The results showed that three SNPs 1113(G/A),9569(G/A)and 9628(G/A)of GDF10 gene were identified in the population.Analysis of association of polymorphism with body measurement traits at all mutation loci showed that there were significant effects on body height,body length,chest circumference and cannon bone girth(P<0.05).There were 8haplotype combinations in the cattle populations,in them AAA and GGA had significant effects on body height,body length,chest circumference and cannon bone girth(P<0.05).The results suggested that three SNPs and two haplotype combinations might have potential effects on production traits in the above mentioned cattle populations and could be used for marker-assisted selection.