和牛超数排卵影响因素的研究

Study on the Influence Factors of Superovulation in Wagyu

为探讨和牛超数排卵的影响因素,试验采用4d8次注射Folltropin-V的超数排卵方法,在不同季节对不同父系和牛供体牛群超数排卵1~10次。结果表明:1春季、夏季、秋季和冬季超数排卵和牛分别获得7.1、7.8、8.3、7.7枚头均胚胎数和5.2、4.7、5.2和5.2枚头均可用胚胎数,差异均不显著(P】0.05);夏季和秋季超数排卵和牛分别获得3.2和3.1枚头均不可用胚胎数,显著高于春季获得的1.9枚头均不可用胚胎数(P【0.05);2和牛供体重复超数排卵1~10次,头均胚胎数、头均可用胚胎数和头均不可用胚胎数分别为7.5~9.3、4.6~6.1和2.7~3.4枚,差异均不显著(P】0.05);3父系为WESFA0107的供体牛群超数排卵获得11.1枚头均胚胎数,显著高于父系为GOSFZ0302、BYWFA0015、BYWFY0342、BYWFY0350和TFWFW06290供体牛群超数排卵获得的头均胚胎数(P【0.05),6.2枚头均可用胚胎数显著高于父系为BYWFY0350的供体牛群超数排卵获得的3.6枚头均可用胚胎数(P【0.05),3.3枚头均退化胚胎数显著高于父系为BYWFA0015、BYWFY0350和TFWFW06290的供体牛群超数排卵获得的1.8、1.1和1.7枚头均退化胚胎数(P【0.05),1.6枚头均未受精卵数显著高于其他6个父系供体牛群超数排卵获得的头均未受精卵数(P【0.05);父系为BYWFY0350的供体牛群超数排卵获得的5.3枚头均胚胎数和3.6枚头均可用胚胎数在7个不同父系供体牛群胚胎生产中均为最少。因此,避开夏季高温季节,增加超数排卵次数同时选择超数排卵反应好的同一父系供体能够提高和牛超数排卵效率。

Experiments were designed to investigate the factors of influencing superovulation in Wagyu.These factors included season,times of repeated superovulation and paternal line.All donors received 120 mg NIH of Folltropin-V in a conatant dose,twice daily IM schedule over 4days.The results showed that:1There were no difference of the mean number of embryos(7.1,7.8,8.3and 7.7)and of transferable embryos(5.2,4.7,5.2and 5.2)in the spring,summer,autumn and winter(P>0.05).The mean number of untransferable embryos(3.2and 3.1)in summer and autumn were significantly higher than in spring(1.9)(P<0.05).2Repeated superovulation(1to 10)was applied to superovulation of Wagyu.There were no difference among the mean number of embryos(7.5to 9.3),of transferable embryos(4.6to 6.1)and of untransferable embryos(2.7to 3.4)(P>0.05).3The mean number of recovered embryos in the WESFA0107line(11.1)was significantly higher than in the GOSFZ0302、BYWFA0015、BYWFY0342、BYWFY0350and TFWFW06290lines(P<0.05).The mean number of recovered transferable embryos in the WESFA0107line(6.2)was significantly higher than in the BYWFY0350line(3.6)(P<0.05).The mean number of recovered degenerate embryos in the WESFA0107line(3.3)was significantly higher than in the BYWFA0015,BYWFY0350 and TFWFW06290lines(1.8,1.1and 1.7)(P<0.05).The mean number of recovered unfertilized ova in the WESFA0107line(1.6)was significantly higher than other lines(P<0.05).The mean numbers of recovered embryos and transferable embryos in the BYWFY0350line(5.3and 3.6)were the least among the paternal lines.In summary,avoiding hot seasons,repeated superovulation and selecting dornors of appropriate paternal line could enhance the production of embryo in Wagyu.