番茄红素对镉诱导睾丸损伤大鼠抗氧化酶活性及生殖激素水平的影响

Influence of Lycopene on Antioxidant Enzyme and Reproductive Hormone in Cadmium-induced Testicular Injury Rats

目的观察番茄红素对镉诱导睾丸损伤大鼠抗氧化酶活性及生殖激素水平的影响。方法将28只雄性SD大鼠随机分为4组,每组7只,分别为空白对照组、5 mg/L镉组、5 mg/L镉+10 mg/kg番茄红素组、5 mg/L镉+20 mg/kg番茄红素组,给药1周后处死,测定睾丸组织超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)活性及血清促黄体生成素(LH)、睾酮(T)水平。结果镉组大鼠体质量、睾丸质量、睾丸组织SOD、GSH-Px活性及血清T水平均显著低于NC组(P<0.01),睾丸组织MDA含量和血清LH水平则显著高于后者(P<0.01);番茄红素可缓解染镉大鼠体质量和睾丸质量的减轻,并回调抗氧化酶活性和生殖激素水平,且高剂量组效果更为显著。结论番茄红素对染镉大鼠的睾丸损伤具有剂量依赖性的改善作用,可能与清除氧自由基和抗脂质过氧化有关。

Objective To observe the influence of lycopene on antioxidant enzyme activity and reproductive hormone level in cadmium-induced testicular injury rats. Methods Twenty-eight SD rats were randomly divided into four groups of equal number: nomal control(NC) group,cadmium(Cd)group,Cd + 10 mg / kg lycopene group,and Cd + 20 mg / kg lycopene group. Then the rats were sacrificed,the activity of Superoxide Dismutase(SOD),Malondialdehyde(MDA),and Glutathione peroxidase(GSH-Px) in testicular tissue,and the level of luteinizing hormone(LH) and testosterone(T) in serum were measured. Results The body weight,testicular weight,SOD and GSH-Px activity,T level of Cd group were significantly lower than those of NC group(P < 0. 01),while MDA and LH level were significantly higher than those of NC group(P < 0. 01). Lycopene can reverse the cadmium-reduced body weight and testicular weight loss,regulate the antioxidant enzyme activity and reproductive hormone level, especially in 20 mg / kg group. Conclusions Lycopene has a dose dependent protective effects against cadmium-induced rat testicular injury,it seems to be closely involved with oxygen free radical scavenging activity and anti-lipid peroxidation