苦味酸分光光度法现场快速测定尿中肌酐

A Rapid Method for Urinary Creatinine Determination by Picric Acid Spectrophotometry

目的建立尿中肌酐的现场快速测定法。方法取尿样20.0μL于10 m L离心管中,用瓶口分配器加入3.02 m L纯水,再加入500μL显色液,于490 nm下,以样品空白溶液校零,测定尿样肌酐含量。结果本方法在质量浓度0 g/L^2.00 g/L呈现良好的线性关系,线性回归方程为C=1.5857*A-0.0112,相关系数达到0.9998,检出限为0.005 g/L,低、中和高三个浓度平均加标回收率为98.6%~99.6%,相对标准偏差为1.6%~2.5%(n=6)。在测定的613份尿样中,测定值为0.08 g/L^4.44 g/L。结论本方法操作简便快速、准确性好、灵敏度高,可用于体检现场快速测定尿肌酐含量。

Objective To establish a rapid method for the determination of creatinine in urine samples. Methods A volume of 20. 0 μL urine sample in a 10 m L centrifuge tube was diluted with 3. 02 m L pure water. After adding 500 μL chromogenic agent and standing for 15 min,the content of creatinine was measured by the absorbance at 490 nm with a blank for zero adjustment. Results The linear range of this method was 0 g / L ~ 2. 00 g /L. The regression equation was C = 1. 5857* A- 0. 0112 and the correlation coefficient was 0. 9998. The detection limit,recovery and relative standard deviation was 0. 005 g / L,96. 9% ~ 104% and 1. 6% ~ 2. 5%,respectively.The content of creatinine in 613 urine samples determined by this method was in the range of 0. 08 g / L ~ 4. 44 g /L. Conclusion The method was rapid,accurate,sensitive,easier to operate and could be used for the determination of urinary creatinine in the field for health checkup.