5株新疆出血热病毒分子流行病学研究

Study on the molecular biology of hemorrhagic fever virus in Xinjiang

目的 从分子水平揭示中国新疆出血热 (XHF)病毒基因结构与功能的关系 ,寻找传播途径及流行原因。方法 对分离自新疆的 5株XHF病毒进行S基因片段的克隆和测序 ,与其他克里米亚 刚果出血热 (CCHF)的S基因序列进行比较和分析。结果 5株病毒S基因全长均为 16 72个核苷酸 ,开放阅读框均为 14 4 9个核苷酸 ,编码一个含 4 82个氨基酸的蛋白。新疆分离毒株的S基因核苷酸序列同源性 (93.0 %～ 99.5 % )明显高于其他国家分离毒株的S基因核苷酸序列。系统发生树状图显示新疆毒株在一个分支之下形成独立的群体 ,明显区别于来自其他地区的CCHF病毒并且又进一步分为三组。结论 不同毒株的S基因序列差异不完全取决于病毒分离的宿主、地域和时间。

Objective To explore the relationship between the structure and function at molecular level and the routes of transmission of Xinjiang hemorrhagic fever(XHF) virus. Methods S genes of five XHF virus strains were cloned, sequenced and compared with that of other Crimean Congo hemorrhagic fever virus strains. Results It was found that S genes of the five viruses had 1 672 nuclei tides, while ORF of them including 1 449 nuclei tides and coded with a protein of 482 amino acid. The nucleotides homology of Chinese isolates ( 93.0 % 99.5 %)was obviously higher than that of any other S genes strains identified in other countries'. Phylogenetic tree showed that all Chinese isolates clustered into one branch and could be further divided into another three groups. Conclusion The sequencial difference of S genes was not totally related to the host, areas and time of the viruses isolated.