响应面法优化蔗糖铁复合物的合成工艺

Optimization of the conditions for the synthetic preparation of iron sucrose complex by response surface methodology

目的通过考察反应温度、反应时间、碱用量和蔗糖投料量4个因素对产物相对分子质量的影响,优化蔗糖铁复合物(ISC)的合成工艺。方法根据单因素实验,选取反应温度(X_1)、反应时间(X_2)、碱用量(X_3)、蔗糖投料量(X_4)作为考察因素,运用凝胶色谱法,以ISC的重均相对分子质量作为考察指标,通过Box-Behnker中心组合设计的响应面法优化ISC的合成工艺。结果反应温度和碱用量对ISC重均相对分子质量有显著影响,4个因素对ISC合成工艺的影响大小为X_3>X_1>X_2>X_4,在所设计的实验条件范围内,升高反应温度和增加碱用量均可降低响应值。结论响应面法优化蔗糖铁复合物制备工艺可用最少的实验获取响应值和变量之间的关系,并得到ISC最佳合成工艺条件。

Objective To optimize the conditions for the synthetic process of iron sucrose complex(ISC),via the investigation of the effects of reaction temperature(X1),reaction time(X2),amount of alkali(X3),and amount of sucrose(X4)on the relative molecular mass of the ISC product. Methods According to the experimental results for the single factor,the conditions dealing with the X1,X2,X3,and X4 parameters for the preparation of ISC were optimized by the Box-Behnker design combined with the response surface methodology using the weight average relative molecular mass of ISC as an indicator,and analyzed with gel permeation chromatography. Results The reaction temperature and the amount of alkali had a significant effect on the weight average relative molecular mass of ISC. The influence of the four factors in the descending order was as follows:X3>X1>X2>X4. In the designed experimental conditions,theresponse value decreased with the increase of both reaction temperatures and alkali amounts. Conclusion Theresponse surface methodology could provide the relationship between the response values and variables via the minimum number experiments to obtain the optimized conditions for the preparation of ISCs.