调经赞育丸对戊酸雌二醇诱导的大鼠无排卵型功能失调性子宫出血的改善作用

Improvement of Tiaojing Zanyu Pills on anovulatory dysfunctional uterine bleeding induced by estradiol valerate in rats

目的探讨调经赞育丸对大鼠无排卵性型功能失调性子宫出血的改善作用及其作用机制。方法将雌性SD大鼠随机分为对照组、模型组。模型组大鼠ig戊酸雌二醇2.8mg/(kg·d),对照组ig等容积生理盐水,连续3周。再将模型组大鼠随机分为模型组、肾上腺色腙组和调经赞育丸155、310、620 mg/kg组,每组各10只。给药组均按10 mL/kg的ig相应药物,对照组、模型组ig等体积的生理盐水,连续7 d。计算各组大鼠的子宫系数,采用酶联免疫吸附法测定大鼠血清中雌二醇和孕酮水平。观察子宫内膜组织病理学形态,并采用免疫组化法检测子宫内膜组织中VEGF表达。结果与模型组比较,调经赞育丸620 mg/kg组大鼠的子宫系数均明显升高(P<0.05);各给药组大鼠血清中雌二醇和孕酮水平均没有明显变化。与模型组比较,调经赞育丸620 mg/kg组大鼠的子宫内膜增生病理改变均明显减轻(P<0.05)。与模型组比较,调经赞育丸310、620 mg/kg组大鼠子宫内膜组织血管内皮生长因子(VEGF)蛋白表达量均明显升高(P<0.05、0.01)。结论调经赞育丸对无排卵型功能失调性子宫出血具有明显的改善作用,其机制可能与升高子宫内膜VEGF蛋白表达量有关。

Objective To investigate the improvement and mechanism of Tiaojing Zanyu Pills on anovulatory dysfunctional uterine bleeding induced by estradiol valerate in rats.Methods Female SD rats were randomly divided into control and model group.Rats in the model group were ig administered with estradiol valerate 2.8 mg/(kg·d).Rats in the control group were given normal saline with equal volume for 3 weeks.Then the rats in the model group were randomly divided into model group,carbazochrome group,and Tiaojing Zanyu Pills(155,310,and 620 mg/kg)group,and each group had 10 rats.The administration groups were given corresponding drugs at the volume of 10 mL/kg,while the control and the model group were given normal saline at the same volume for 7 d.Uterine coefficients of rats in each group were calculated.Serum estradiol and progesterone levels were measured by ELISA.The histopathological morphology of endometrium was observed,and the VEGF expression in the endometrium was detected by immunohistochemistry.Results Compared with the model group,the uterine coefficient in Tiaojing Zanyu Pills(620 mg/kg)group were significantly increased(P<0.05).The levels of estradiol and progesterone in each group were not significantly changed.Compared with the model group,the pathological changes of endometrial hyperplasia in Tiaojing Zanyu Pills(620 mg/kg)group were significantly alleviated(P<0.05).Compared with the model group,the VEGF expression in Tiaojing Zanyu Pills(310,620 mg/kg)group were significantly increased(P<0.05,0.01).Conclusion Tiaojing Zanyu Pills has a significant effect on improvement of anovulatory dysfunctional uterine bleeding,and its mechanism may be related to increasing expression of VEGF in the endometrium.