精索静脉曲张大鼠环磷酸腺苷反应元件调控因子表达与睾丸生精功能损害的关系

The correlated study on CREM expression and testicular breeding sperm functional lesion in rats with varicocele

目的 探索精索静脉曲张导致男性不育的机理。方法 选择 30只SD大鼠 ,2 0只作为手术组 ,建立精索静脉曲张模型 ;10只作为假手术对照组。 3个月后 ,取下睾丸常规染色切片 ,用Makler积分法分别测量手术组 2 0 0个 ,对照组 10 0个曲细精管内径、管周膜厚度、细胞层数及生精细胞成熟程度 ,并计算平均得分 ;原位杂交方法测定CREM的表达量 ,并比较二组之间的差异 ,分析环磷酸腺苷反应元件调控因子 (CREM)表达与生精功能损害的相关性。结果 与对照组比较精索静脉曲张组的曲细精管内径显著缩小 (10 1 2± 2 2与 146 0± 4 1) ,管周膜增厚 (3 5± 0 1与 1 9± 0 2 ) ,细胞层次减少 (3 0± 0 2与 5 5± 0 1) ,生殖细胞成熟障碍 (3 6± 0 3与 4 9± 0 1) ,Makler评分显著低于假手术组 (16 0± 1 2与 8 5± 0 6 ) ,二者有非常显著的差异。CREM在曲张组表达显著减少 (曲张组2 0± 0 32 ,假手术组 3 90± 0 32 ) (P <0 0 0 1) ,且表达定位于精原细胞至精母细胞阶段 ,其表达量与评分值正相关 (r=0 746 ,P <0 0 0 1)。结论 精索静脉曲张睾丸组织中CREM表达减少可导致生精功能损害 ,可能是导致不育的原因之一。

Objective To study male infertility′s mechanism caused by varicocele. Methods 30 rats were selected at random,of 20 were operated,as varicocele′s group;of 10 were pseudo-operated,as controlled group, testes were removed, stained and sected.Makler Score were adopted to analyse the bore,limitan′s thickness,layer number of cell,the dregree of cell′s maturity and average score in 200 and 100 seminiferous tubulae respectively.CREM was determinated by hybridization in situ,the difference between two groups was compared. Results In varicocele′ group,seminiferous tubule′s bore was decreased (101.2±2.2 vs 146.0±4.1 ),limitan became thicker (3.5±0.1 vs 1.9±0.2) ,cell′ s layer number was reduced (3.0±0.2 vs 5.5±0.1), cell′s maturity turned to disturbance (3.6±0.3 vs 4.9±0.1),the average score was lower than controlled group (16.0±1.2 vs 8.5±0.6),they had significant differences (P<0.001).CREM expression was lower in varicocele group than in controlled one (VG 2.0±0.32,SoG 3.90±0.32)(P<0.001), which was located in spermatogenous cell to spermatocyte stage.The quantu of expression and score value brought out positive correlation (r=0.746, P<0.001). Conclusions CREM lower expression could lead to breeding sperm functional lesion,which probably was one of the mechanisms of infertility caused by varicocele.