庚型肝炎病毒(HGV RNA)基因的定量检测

Quantitative detection of hepatitis G virus gene

目的 :建立庚型肝炎病毒基因 (HGVRNA)的定量检测方法。方法 :采用荧光转换原理 ,应用特异荧光物质标记的引物进行逆转录PCR(RT_PCR) ,检测 4 1例临床血清标本的HGVRNA。结果 :与巢式定性PCR结果比较 ,两者具有相关显著性。以定量实验为标准定性实验的阳性漏检率为 3.33% ,阴性漏检率为 18.1% ,两者相对符合率为 92 .6 8%。结论 :定量检测HGVRNA在定性的基础上提供了量的结果 ,而且具有特异性强、结果稳定。

Objective:To establish an assay for quantitative detection of hepatitis G virus gene.Methods:Based on the principle of fluorescence energy transfer, the assay enables a homogeneous detection format to allow concurrent detection and amplification in a single reaction vessel. Forty one samples of clinical sera were examined by quantitative PCR amplification. Results:It was shown that data of quantitative detection had a remarkable correlation with that of nested_PCR results. When the quantitative detection served as the standard, the rates of missed positive and missed negative were 3.33% and 18.1% respectively with qualitative detection. The coincidence rate between these two methods was 92.68%. Conclusions:The quantitative detection of HGV provides the quantitative results on the basis of qualitative results, and is specific, simple and stable.