芽孢杆菌组合对海水养殖水体COD的降解效果

Study on COD Degradation Capability of Bacillus Combinations

【目的】获得一种降解性能优于单菌株的菌株组合,更好地改善目前工业化密度养殖的水体水质。【方法】从刺参(Stichopus japonicus)养殖池塘分离降解效果良好的单菌株,根据菌株之间的拮抗效应,按照2株菌或3株菌进行组合,研究各组合菌株的降解效果。【结果】2株菌的组合第6天时COD含量的均值为422.57mg/L,标准差为63.85,3株菌的组合第6天时COD含量的均值为365.61mg/L,标准差为67.63,说明3株菌的组合整体降解效果比2株菌的组合好,且更容易获得降解效率高的组合,但并不是所有3株菌的组合降解效果都优于2株菌的组合。【结论】确定最优菌株组合为C14(菌株B2,菌株B6,菌株B11),COD降解率为64.29%。经鉴定,菌株B2为枯草芽孢杆菌(Bacillus subtilis),菌株B6为巨大芽孢杆菌(Bacillus megaterium),菌株B11为嗜碱性芽孢杆菌(Bacillus alcalophilus),为今后的实践应用提供参考。

【Objective】To improve the water quality in high stocking density of industrial aquaculture,a kind of strain combination,with degradation capability better than single strain,was found.【Methods】Several bacterial strains with good degradation property were separated fromStichopus japonicus culture ponds.Antagonistic effect between strains was studied.According to the results of the antagonistic effect,the degradation capability of COD of the combinations of 2or 3strains were studied.【Results】The mean COD of the combinations of two strains at the 6th day was 422.57mg/mL,and standard deviation was 63.85.The mean COD of the combinations of three strains at the 6th day was 365.61g/mL and the standard deviation was 67.63.The results showed that combinations of three strains compared to combinations of two strains were easier to get a combination with better degradation efficiency.【Conclusion】Finally,the optimal combination was C14(strain B2,strain B6,strain B11)with COD degradation rate of 64.29%.The strain B2,strain B6 and strain B11 were separately identified as Bacillus subtilis,Bacillus megaterium and Bacillus alcalophilus.The result was a reference for future applications of strains.