植物磷酸化蛋白质组研究进展

Research Progresses on Phosphorylation of Plant Phosphoproteome

蛋白质磷酸化是蛋白质翻译后修饰研究最深入的领域之一,蛋白质磷酸化也是信号转导的关键调控因子。磷酸化修饰可精细地协调激酶和磷酸化酶网络,进而调控细胞生长、增殖及胞内外生物刺激应急反应等。但是由于蛋白质磷酸化短暂性、高动态性及低丰度的特点,使得磷酸化蛋白质组研究具有挑战性。本文从基于2-DE的植物磷酸化蛋白质组、植物磷酸化肽富集、基于质谱植物磷酸化肽鉴定及位点分析、基于质谱植物磷酸化肽定量分析、植物磷酸化蛋白质数据库等方面综述植物磷酸化蛋白质组研究进展、策略、相应技术的优缺点及展望了未来发展的方向。

Protein phosphorylation is one of the most studied fields of protein post-translational modification as well as the key factor to control the signal transduction. Phosphorylation can fine coordinate the networks kinase and phosphorylase to further regulate cell growth, proliferation and stress responses to intracellular biological stimulation etc. However, due to the characteristics of transient, high dynamic and low abundance of protein phosphorylation, the study of phosphoproteome makes deeply challenges This paper reviewed the progresses and strategies on plant phosphoproteome including the 2-DE based plant phosphoproteome, phosphopeptide enriched plants, site identification and analysis of plant phosphorylation peptide based on mass spectrometry, plant phosphorylation peptide quantitative analysis based on mass spectrometry, plant phosphorylation protein database etc. and commented the advantages and disadvantages of plant phosphorylation technologies and prospected the future direction on this research field.