不同营养状况下血清瘦素与家兔肝功能的关系研究

Relationship between Serum Leptin and Liver Function of Rabbits under Different Nutritional Conditions

家兔的瘦素水平及肝功能受营养状况的影响,为研究三者的关系,将健康雌性新西兰白兔(40日龄)随机分为三组:正常对照组、过饲组及限饲组,每组10只。按营养标准正常饲喂的设为正常对照组,以高于对照组30%的日饲喂量作为过饲组,低于对照组30%的日饲喂量作为限饲组。各组检测指标:体重、血清瘦素(leptin)水平、总蛋白(TP)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆汁酸(TBA)、总胆红素(T-BIL)、脂肪细胞中的瘦素水平及脂肪细胞的瘦素m RNA相对表达量。结果为:12周末,过饲组和限饲组的体重均发生显著变化(p<0.05),且两组的血清Leptin均显著高于对照组(p<0.05);12周末过饲组和限饲组脂肪细胞中的leptin水平均显著高于对照组(p<0.05);过饲组和限饲组脂肪瘦素的m RNA表达均显著高于对照组,而且限饲组也显著高于过饲组(p<0.05);限饲组血清谷丙转氨酶、谷草转氨酶、总胆汁酸、总胆红素均高于对照组和过饲组(p<0.05);限饲组血清总蛋白水平显著低于对照组和过饲组(p<0.05)。结论:营养不良可引发血清瘦素水平升高并伴随出现肝功能受损。但在正常饲喂和营养供给充分的情况下,动物的肝功能未见异常,且在一定的生长时期内体重越大,血清瘦素水平越高。

Leptin and liver function of rabbits are affected by nutritional status. In order to study the relationship between them, healthy female New Zealand rabbits(40 days old) were randomly divided into 3 groups: control group(n=10) which was fed with normal diet, excessive feeding group(n=10) and restricted feeding group(n=10).Excessive feeding group was fed with diet 30% more than the control group. On the contrary, restricted feeding group was fed with diet 30% less than the control group. Weight, serum leptin level, total protein(TP), leptin levels of fat cells, adipocyte leptin m RNA relative expression and liver function index including ALT, AST, TBA and T-BIL were performed. Weight in groups including excessive feeding group and restricted feeding group had changed significantly and serum leptin were significantly higher than those in control group(p<0.05) after 12 weeks. At the same time, leptin levels of fat cells and adipocyte leptin m RNA relative expression were higher than those in control group(p<0.05). In addition, adipocyte leptin m RNA expression in restricted feeding group were even significantly higher than those in excessive feeding group(p<0.05). Levels of ALT, AST, TBA and T-BIL in restricted feeding group were significantly higher than those of control group and excessive feeding group(p<0.05).However, levels of TP in restricted feeding group were significantly lower than those in groups including control group and excessive feeding group(p<0.05). In summary, malnutrition coulld cause abnormal levels of serum leptin and liver function. But liver function was normal and the greater weight caused the higher level of serum leptin under the conditions of normal and sufficient feeding.