牛血清蛋白与纳米金颗粒相互作用的研究

The Study on the Interaction between Bovine Serum Albumin and Gold Nanoparticles

为了研究纳米颗粒与蛋白质的相互作用原理,我们选取纳米金颗粒及牛血清蛋白为材料,通过牛血清蛋白与纳米金颗粒的物理吸附作用,结合蛋白酶K酶切、SDS洗脱以及SDS-聚丙烯酰胺凝胶电泳检测的方法,我们了解到,纳米颗粒与蛋白质的结合主要是由蛋白质中的某一段特定的序列结合在纳米颗粒的表面所导致的,该结果的发现不仅对纳米颗粒在生物体内广泛的应用奠定了理论基础,对功能性纳米颗粒在生物体内功能的发挥也是至关重要的。

In order to study the interaction between bovine serum albumin and gold nanoparticles, we selected gold nanoparticles and bovine serum albumin as material, thought the bovine serum albumin and physical adsorption with gold nanoparticles, combined with proteinase K digestion, SDS elution and SDS-polyacrylamide gel electrophoresis, we learned that the proteins combine with the nanoparticles primarily due to a segment of the protein sequence specific binding to the surface of the nanoparticles. We found this resule not only laid the theoretical foundation of the nanoparticles widely used in vivo, but also critical for functional nanoparticles play function in vivo.