摘要
国内外毛细管电泳(CE)产品深受电泳焦耳热存在的影响,使得毛细管电泳试样的流通量非常少,严重影响到了CE发展与应用。本文对毛细管电泳技术存在的问题进行进一步的量化分析,利用智能控制技术来构造微管电泳检测系统,采用内制冷的方式,及时原地的带走电泳过程中产生的焦耳热,采用多通道高压电源作为整个电泳系统的驱动力,通过对运行缓冲液的合理调控,实现对待分离组分的高效分离。研究出高效微管电泳技术的全分析系统模型,以期能给生命科学、材料科学、环境科学提供又一强有力的研究手段。
Capillary electrophoresis(CE) products at home and abroad, deeply influenced by the existent electrophoresis of joule heat, with result of little circulation of the capillary electrophoresis sample, greatly impact the development and application of CE. In this paper, the further quantitative analysis on problems existing in the capillary electrophoresis technology, and take away the joule heat promptly that generated in the process of the electrophoresis through the intelligent control technology and inner refrigeration method. The technology uses the multichannel high voltage power as the driving force of the whole system and achieves the efficient separation of components by reasonable control of the running buffer. The model of the efficient micro capillary electrophoresis technology analysis would provide another powerful method for the research of the bioscience and material science and environmental science.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第5期1067-1070,共4页
Genomics and Applied Biology
基金
西京学院校级科研项目(XJ120227)资助
关键词
微管电泳
毛细管电泳
焦耳热
智能冷却
检测技术
Microtubule electrophoresis,Capillary electrophoresis,Joule heat,Intelligent cooling,Detection technology