Ang-(1-7)通过G蛋白偶联受体Mas在人肝癌细胞HepG2中的作用

The Effect of Ang-(1-7) on Human Hepatocellular Carcinoma Cells: HepG2 by G Protein Coupled Receptor Mas

为了研究Ang-(1-7)通过Mas对人肝癌细胞增殖的影响及在Mas高、低表达模型中分析其表达差异、凋亡等细胞生理活性变化,我们采用药物(Mas激活剂Ang-(1-7),Mas抑制剂A779)干预Hep G2细胞,分别对Ang-(1-7)和A799进行剂量、时间效应实验,实时定量RT-PCR检测药物干预后细胞Mas的表达水平,最后流式细胞仪检测药物干预后细胞的凋亡变化。结果表明,Ang-(1-7)与A779的有效工作浓度为10-7mol/L,Ang-(1-7)促进Mas的表达,Mas表达水平高于对照组。相反,A779抑制Mas的表达,但作用不明显,主要为拮抗Ang-(1-7)的功能。Ang-(1-7)处理组细胞凋亡明显升高,揭示Mas表达提升诱导人肝癌细胞凋亡。

To investigate the effect of Ang-(1-7) on the proliferation of human primary hepatocellular carcinoma(HCC) cells by Mas and analyze the expression differences in physiological activity and cell apoptosisthe in Mas(high and low) model, we applied drugs intervention on Hep G2 cells. Determining the effective concentration by the experiment of dose effect and dose dependence experiment, apply Real-time quantitative RT-PCR to detect the expression level of mas after drug intervention and detect the changes of cell apoptosis by flow cytometry instrument. As the results, the effective concentration of Ang-(1-7) and A779 were both 10-7mol/L. Ang-(1-7) can promote the expression of mas, in the RT-PCR the expression level of mas was higher than the control which was opposite of A779. Up-reguluted mas promoted the apoptosis of human hepatocarcinoma cells obviously.