大豆miR172家族成员序列及表达模式初步分析

Sequence and Expression Pattern Analysis of miR172 Family in Soybean

Micro RNAs(miRNAs)在植物的生长发育以及植物对环境的适应方面发挥了重要的作用,成为近几年研究的热点。mi R172家族是一个保守的mi RNA家族,之前的研究表明mi R172的作用涉及生长期过渡、闭花受精、花器官发育、开花时间调控等方面。但是到目前为止,人们对大豆mi R172家族的了解还不是很清楚。本实验通过mi RBase、PLACE数据库及DNAman软件对gma-mi R172家族成员的前体序列、成熟序列、启动子序列进行比较分析,发现gma-mi R172家族成员成熟序列虽然高度保守,但前体序列具有一定的差异,这可能导致了各成员的功能差异;启动子分析显示其启动子含有光反应原件和多种非生物胁迫响应元件,推测其可能参与大豆的开花调控和逆境胁迫响应。通过实时定量PCR分析了大豆mi R172成员的组织特异性表达情况。利用PMRD数据库对gma-mi R172的靶基因进行了生物信息学分析,获得了7个AP2-like类转录因子,参与花器官发育、开花时间调控及非生物胁迫响应的方面。上述结果初步分析了大豆mi R172家族的功能与靶基因,对今后进一步分析研究大豆mi R172家族及其靶基因打下了基础。

Micro RNAs(mi RNAs) play crucial roles in many aspects of plant development and responses to the environment, it has become one of popular research topic recent years. The mi R172 family is a conserved mi RNA,they have been extensively studied in growth-phase transition, cleistogamy, flower development and flowering-time control in previous studies. But so far, the function of gma-mi R172 is not clear to us. In the present study, the premi RNAs sequence, mature sequence and promoter of gma-mi R172 were compared and analyzed through mi RBase,PLACE databases and DNAman. Analysis results showed that the sequence similarity between mature mi R172 family members is very high, but it varies among the pre-mi RNAs, this may caused the functional differences between the members. Promoter analysis showed that many important photoreaction and abiotic stress cis-acting regulatory elements were found in the promoter sequence, speculating it may participate in soybean flowering regulation and adversity stress response. We analyzed the expression profile of gma-mi R172 in different tissues. Seven AP2-like transcription factor target genes of gma-mi R172 were identified by PMRD software, which involved in flower development and flowering-time control and abiotic stress responses. These results preliminary reveals the function of gma-mi R172, laying foundation for the future analytical investigation of gma-mi R172 and their target genes.