水稻(Oryzasativa L.)潜在内源性致敏原的生物信息学筛选与分析

Bioinformatic Screening and Analysis of Potential Endogenous Allergens in Rice(Oryza sativa L.)

食物中的蛋白质是最常见的食入性致敏原。为了对水稻中的内源性致敏原有一个全面的认识和了解,本研究利用全序列比对的方法比较了水稻蛋白与已知致敏原之间的序列相似性,获得了水稻潜在致敏原,并对其保守结构域、分子功能、系统发育和染色体分布进行了分析和描述。结果一共发现了122个水稻潜在致敏蛋白,其中包括了22个已被数据库收录的水稻致敏原;保守结构域分析显示它们分别属于37个蛋白家族,其中的HATPase_c_3(Histidine kinase-,DNA gyrase B-,and HSP90-like ATPase,PF13589)结构域未在已知致敏原中发现;系统发育分析则分别揭示了水稻Prolamin蛋白超家族和Dna K蛋白家族的多样性;而且潜在致敏蛋白在水稻染色体上有成簇状分布的趋势。

The protein in the food is the most common allergic component. In order to have a clear understanding of endogenous allergens in rice, full length sequence alignment was performed to compare the similarities between rice proteins and known allergens. Potential allergens in rice were obtained and characterized in conserved domain, molecular function, phylogenetic relation and distribution among the chromosomes. Results showed that122 potential rice allergens were identified and characterized by bioinformatic methods, including the 22 allergens in present databases. Conserved domain analysis revealed 37 domains among the potential rice allergens including one novel domain(Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase, PF13589) adding to the allergen protein database. Phylogenetic analysis of the potential allergens revealed the diversity among the Prolamin superfamily and Dna K protein family, respectively. Additionally, some potential allergenic proteins clustered on the rice chromosome might suggest the molecular function during the evolution.