摘要
低能离子注入技术和方法已广泛应用于微生物诱变育种,为了进一步理解低能N+注入原核微生物与真核微生物细胞的离子输运过程与效应,本文根据腊样芽孢杆菌和酿酒酵母菌的化学组成与细胞结构,建立了两种简化的微生物靶材料模型,利用Monte-Carlo方法计算了能量15 Ke V的N+注入在两种不同类型微生物细胞中的离子射程分布、径迹结构、能量沉积及靶损伤情况,比较了N+注入对微生物细胞的损伤效应,对芽孢杆菌死亡率高于酵母菌死亡率的机理进行了理论分析。
The techniques and methods of the low-energy ion implantation have been widely used in microbial breeding. In order to further understand the transport process and effect of prokaryotic and eukaryotic microbial cell by N+implantation, the simple biological targets material models were established which based on the chemical composition and cell structure of Bacillus cereus and Saccharomyces cerevisiae. By Monte-Carlo method when 15 Ke V N+implanted in the two types of microbial cells, the ion range distribution, track structure, energy deposition and target damage were simulated and calculated, and the damage effects were compared, and the mechanism of B.cereus mortality higher than S. cerevisiae was analyzed in this paper.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第8期1729-1733,共5页
Genomics and Applied Biology
基金
国家自然科学基金(11275164)资助
