应用RAMP标记分析国产姜黄属植物的遗传多样性

Genetic Diversity of Chinese Curcuma based on RAMP Makers

本研究运用RAMP(random amplified microsatellite polymorphism)分子标记分析了10种国产姜黄属(Curcuma)植物的遗传变异和亲缘关系。结果得到520条迁移率不同的条带,其中多态性条带有509条,每条引物平均得到10.6条带和10.4条多态性带,多态性条带的百分率为97.88%。69份供试材料之间遗传相似系数最大值为0.921,最小值为0.550。当遗传距离是0.61时,可将其分为四大类群。研究结果表明种间关系与地理位置有一定的关系,同时证实了姜黄属植物种质资源遗传多样性丰富,为姜黄属植物的分类鉴定奠定了基础。

Relationship and genetic variation among ten species of Chinese Curcuma were analyzed by using RAMP(random amplified microsatellite polymorphism) markers. A total of 520 bands were amplified based on selected 49 primers and each primer obtained an average of 10.6 bands. Meanwhile, 509 products were found to be polymorphism and each primer obtained an average of 10.4 polymorphic bands. The GS maximal value among all the materials was 0.921 when the minimal was 0.550. When the genetic distance was 0.61, the 69 materials could be divided into four categories according to the cluster analysis. The results exhibited a certain relationship between the genetic relationship among the species and geographical location and verified that Curcuma germplasms would have abundant genetic diversity that may lay the foundation of classification and determination.