小鼠孤雌激活来源囊胚线粒体基因表达

Expression of Genes in Mitochondria Derived from Parthenogenetic Embryo in Mice

为了探索孤雌胚胎和正常体外受精胚胎线粒体基因表达的差异,本研究用ICR小鼠孤雌和正常受精胚胎为研究对象。孤雌和正常受精的胚胎分别发育至2-细胞期和囊胚期的比率,用q PCR方法检测囊胚线粒体基因Cox2、tom40、tim23和cytochrome C,以及多能性囊胚质量相关基因Oct4、Sox2和nanog的表达水平。结果发现孤雌激活不影响小鼠卵母细胞的卵裂(95%vs 97.6%,p>0.05),但影响胚胎发育到囊胚(39.4%vs 75%,p<0.05),孤雌囊胚细胞线粒体Cyto C和Cox2显著高于体外受精组(p<0.05);正常受精囊胚多能性基因Oct4和nanog表达水平显著高于孤雌组(p<0.05),但Sox2表达水平显著低于孤雌组(p<0.05)。本研究表明孤雌激活可影响胚胎线粒体和细胞基因表达水平。

To invest igate the genes expression differences in mitochondria between blastocysts derived from parthenogenetic and fertilized embryo, the outbred strain ICR mice was used to do it. The capabilities of development to 2-cell and blastocyst was compared between parthenogenetic and fertilized groups. The gene expression level of Cox2, tom40, tim23 and cyto C related to mitochondria and Oct4, Sox2 and nanog related to Pluripotent and blastocyst quality was detected by q PCR method. The results showed that capability of cleavage stage was not influenced by artificial activation, compared with fertilized group, but that of blastocyst was affected. The expression level cyto C and cox2 specialized to mitochondria in parthenogenetic group was more than that of fertilized group. Expression level of Oct4 and nanog in fertilized group was higher than that of parthenogenetic group, contrary to which, sox2 was significant lower in fertilized group, compared to parthenogenetic group. The research demonstrated that parthenogenetic activation could impose the gene expression in mitochondria and cells in embryo.