木薯IPT基因的序列分析及其乙烯和茉莉酸甲酯诱导表达特性

Sequence Analyzing of IPT Gene and Expression Characteristic Induced by Ethylene and Methyl Jasmonate in Manihot esculenta Crantz

木薯是一种重要的能源和淀粉作物,具有较强的抗逆性。为研究木薯中决定细胞分裂素合成的IPT基因如何被逆境信号调控,从木薯基因组数据库中获得了两个Me IPT基因,序列分析显示两者启动子区同时具有茉莉酸和乙烯响应元件。以木薯品种"华南八号"悬浮培养细胞为材料,利用q RT-PCR检测木薯Me IPT1和Me IPT2基因在乙烯利和茉莉酸甲酯处理后的表达模式。结果显示茉莉酸甲酯处理后两个基因显著上调,但乙烯信号对其的影响却不尽相同。这些数据说明这两个Me IPT基因可被乙烯和茉莉酸信号调控,并推测其在进一步影响细胞分裂素的生物合成中可能受整合后的不同激素信号调控。

Cassava(Manihot esculenta Crantz) is a kind of important energy and starch crop, with strong stress resistance. To study how the gene IPT determining biosynthesis of cassava cytokinin was regulated by stress signals,two Me IPT gene sequences were extracted from cassava genome database, and sequences analysis showed that two Me IPT genes both had jasmine acid and ethylene responsive elements in promoter regions. Using suspension cultured cassava 'SC8'cells as material, expression patterns of Me IPT1 and Me IPT2 were tested by quantitative real time PCR after treated by ethephon and methyl jasmonate. The results showed that two genes were up-regulated significantly after treated with methyl jasmonate, whereas the influences of ethylene signal to them were not same. These data suggested that two Me IPT genes could be regulated by ethylene and jasmine acid signals, and presumed that their further affection on biosynthesis of cytokinin might be regulated by integrated different hormones stimulation.