摘要
在Gen Bank中找到粉质胚乳突变基因Mucronate,构建以bar基因为筛选标记的植物表达载体p CAMBIA3300-Ltp2-Zm MC。通过农杆菌介导的萌动胚转化法将Ltp2-Zm MC基因转入玉米自交系郑58中,用含5 mg/L草胺磷的筛选培养基进行筛选,共获得280株再生植株。经过PCR和RT-PCR鉴定,得到29株RT-PCR阳性植株。结果表明,Zm MC基因在玉米中得到表达。
The mutant gene Mucronate(Mc) of farinaceous endosperm was found according to Gen Bank, and the plant expression vector p CAMBIA3300-Ltp2-Zm MC with the bar gene as the selection marker was constructed.Ltp2-Zm MC gene was transformed into germinating embryo of maize inbred line Zheng 58 by Agrobacterium-mediated transformation, screening with selection medium containing 5 mg/L of PPT to obtain a total of 280 PPT-resistant seedlings. After PCR and RT-PCR identification, 29 plants were positive. The results showed that the Ltp2-Zm MC gene was expressed in maize.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第12期2690-2694,共5页
Genomics and Applied Biology
基金
吉林省科技厅产业技术创新战略联盟项目-新型雄性不育化玉米种子生产技术(20140309005NY)
国家支撑计划项目-春玉米区商业化育种技术研究与示范(204BAD01B01)共同资助
