草莓光信号转导因子FaHY5的克隆及其表达分析

Cloning and Expression Analysis of Light Responsive bZIP Transcription Factor FaHY5 from Strawberry

以‘丰香’草莓为试材,通过同源克隆法获得FaHY5 cDNA全长序列,运用生物学软件对该序列及其编码的氨基酸序列进行相关生物信息学及结构功能分析,同时采用半定量及荧光定量方法对FaHY5基因在不同组织和果实不同发育阶段的表达模式进行研究。结果表明,FaHY5全长为501 bp,Gen Bank登录号为KP984791,编码166个氨基酸。蛋白质分子量约为18.136 kD,理论等电点为10.05,C末端存在典型的bZIP保守结构域,是一个非分泌型核定位蛋白。序列比对及进化树分析,HY5在进化的过程中具有高度的保守性。表达谱分析,FaHY5在各组织中均有表达,且在花中表达量最高;而在果实发育过程中,FaHY5主要在前期积累。本研究分离鉴定了草莓中HY5(LONG HYPOCOTYL 5)转录因子并探索了其在草莓中的表达模式,为进一步研究草莓FaHY5转录因子的功能和作用机制提供理论基础。

Fa HY5 gene was cloned from strawberry(Fragaria×ananassa cv. Toyonaka) though homology cloning method. Then, Bioinformatics and expression pattern of Fa HY5 were analysed by some softwares and PCR methods, respectively. The full length of Fa HY5 c DNA is 501 bp and Gen Bank accession number is KP984791. It encodes a protein consist of 166 amino acids residues with a molecular mass of 18.136 KD and a p I of 10.05. The protein contains the b ZIP conserved domain, which was located in nucleus and has no signal peptide. Sequence alignment and phylogenetic analysis, HY5 is highly conserved in evolution process. Gene expression profiling,Fa HY5 expresses among all different tissues, and the highest expression level is detected in flower. During fruit development, Fa HY5 accumulates mainly in the early stages. This research isolated and identificated light responsive b ZIP Transcription Factor HY5(LONG HYPOCOTYL 5) from strawberry and analysed gene expression pattern, which may provide a theoretical basis for further study of the function and molecular regulation mechanism.