bFGF调控成釉细胞中牙釉质基质蛋白的基因表达

Basic Fibroblast Growth Factor Regulates Gene Expression of Enamel Matrix Proteins in Ameloblasts

本研究旨在说明b FGF可以通过DLX1/DLX2调控成釉细胞中牙釉质基质蛋白的基因表达。首先利用免疫组织化学显示DLX1/DLX2在分泌期成釉细胞中的表达;分离培养成釉细胞,并通过RT-PCR检测Dlx1/Dlx2及牙釉质基质蛋白在成釉细胞中的表达;在成釉细胞中分别过表达Dlx1和Dlx2后,Real time PCR检测牙釉质基质蛋白表达的改变;最后以50 ng/m L的b FGF的刺激成釉细胞,研究b FGF对Dlx1/Dlx2表达的影响。结果显示DLX1在分泌期小鼠成釉细胞中表达信号呈强阳性,而DLX2较弱;成功分离培养成釉细胞后,RT-PCR证实了Dlx1/Dlx2与牙釉质基质蛋白基因的表达在时空上存在同步性。在成釉细胞中分别过表达Dlx1和Dlx2对Ambn、Amelx、Amtn和MMP20的表达均有不同程度的影响;进一步研究发现b FGF能够促进Dlx1和Dlx2的表达。本研究结果提示b FGF可以通过DLX1/DLX2调控成釉细胞中牙釉质基质蛋白的基因表达。

The aim of this study was to show that b FGF can regulate the gene expression of enamel matrix proteins in enamel cells by DLX1/DLX2. Firstly, the expression of DLX1/DLX2 in ameloblasts during secretory phase was revealed by immunohistochemistry. Ameloblasts were isolated and cultured, and the expression of Dlx1/Dlx2 and enamel matrix proteins in ameloblasts was detected by RT-PCR. After Dlx1 and Dlx2 were over expressed respectively in ameloblasts, expression of enamel matrix proteins was detected by Real time PCR. Finally, the effect of b FGF on the expression of Dlx1/Dlx2 in ameloblasts was studied by 50 ng/m L b FGF stimulating cells. The results showed that the expression of DLX1 was strongly positive, while DLX2 was weak in ameloblasts during secretory phase. After successful isolation and culture of the cultured cells, RT-PCR confirmed that the expression of Dlx1/Dlx2 and enamel matrix protein gene was synchronous in time and space. The expression of Dlx1 and Dlx2 had different effects on the expression of Ambn, Amelx, Amtn and MMP20, respectively. Further study found that b FGF can promote the expression of Dlx1 and Dlx2. The results of this study suggest that b FGF can regulate gene expression of enamel matrix proteins in ameloblasts by DLX1/DLX2.