慈竹BeSUS1基因克隆与表达分析

Cloning and Expression Analysis of BeSUS1 Gene in Bambusa emeiensis

从慈竹转录组数据库中克隆得到了一个SUS基因,命名为Be SUS1,进行了生物信息学分析和组织表达分析。本研究表明,该基因该序列长为2 536 bp,ORF框长度为2 451 bp,编码816个氨基酸,具有N端的蔗糖合成功能域和C端的糖基转移功能域,Be SUS1属于单子叶SUS一簇,与绿竹、水稻、玉米等氨基酸序列,理化性质以及蛋白结构具有高度的同源相似性。组织表达分析显示Be SUS1在慈竹各个部位均有表达,但在各组织中表达具有一定差异,其在未展开叶中表达较低,而在成熟叶与茎段组织中表达较高。

According to the transcriptome data of Bambusa emeiensis Chia et H. L. Fung 'Viridiflava' shoots, one SUS genes designated as Be SUS1 was cloned and its bioinformatics were analyzed. The full-length of nucleotide sequence was 2 536 bp, containing a complete open reading frame with 2 451 bp, encoding 816 amino acids, and having an N-terminal domain of sucrose synthetase and C-terminal domain of a glycosyltransferase. Phylogeny analysis showed the Be SUS1 was falled into monocot SUS group. Amino acid sequence alignment, physiological characteristics analysis and tertiary structure analysis showed Be SUS1 was highly homologous to Bo SUS1, Bo SUS3 and Os SUS1. Furthermore, the transcript of Be SUS1 gene was subsequently examined. Be SUS1 was expressed in all parts of Bambusa emeiensis, but in different tissues had different expression. It was predominantly expressed in mature leaves and stem while very slightly in unexpanded leaves.