桉树焦枯病菌MAPK的鉴定与生物信息学分析

Identification and Bioinformatics Analysis of Cyliudrocladium Leaf Blight MAPK of Eucalyptus

利用多种生物信息学网站与软件,从全基因组水平鉴定桉树焦枯病菌的促分裂原活化蛋白激酶(MAPK),并对其理化性质、亚细胞定位、结构域、二级结构、三级结构等进行分析,并构建系统发育树。结果表明,在桉树焦枯病菌中发现4个MAPK,分别命名为CpKss1、CpSlt2、CpHog1和CpIme2,均属于亲水蛋白,亚细胞定位均在细胞核。拥有高度保守的结构域STKc及位于活化环上的双重磷酸化位点T-X-Y,三级结构显示拥有蛋白特异性底物结合口袋特征。通过系统进化分析,这4个MAPK相应基因分别与酵母Fus3/Kss1、Slt2、Hog1和Ime2类基因同源,均和禾谷镰刀菌中相应的MAPK亲缘关系最近。该研究结果为进一步了解桉树焦枯病菌中MAPK的生物学功能和构建MAPK级联途径奠定基础。同时对桉树焦枯病菌致病、桉树抗病相关重要基因功能及分子机制等研究具有重要意义,最终为桉树焦枯病防治工作提供技术支持。

Using a variety of bioinformatics websites and software,the mitogen-activated protein kinase(MAPK)of C.Pseudoreteaudii from the wh ole genome level was identified,and its physical and chemical properties,subcellular localization,domain,secondary structure and tertiary structure were analyzed,as well as the phylogenetic tree was constructed.The results showed that four MAPKs were identified in C.Pseudoreteaudii and named CpKss1,CpSlt2,CpHog1 and CpIme2,respectively,which were all hydrophilic protein and their subcellular localization were located at nucleus.The four MAPK had a highly conserved domain STKc and a double phosphorylation site T-X-Y located on the activation ring.The tertiary structure showed the characteristics of protein-specific substrate-binding pockets.Through phylogenetic analysis,the corresponding genes of four MAPKs were homologous to the genes of yeast Fus3/Kss1,Slt2,Hog1 and Ime2,respectively,and they also had the closest relationship with the corresponding MAPK in Fusarium graminearum.The results would lay a foundation for further understanding the biological function of MAPK in C.Pseudoreteaudii and constructing the pathway of MAPK cascade.At the same time,it would be of great significance to study the pathogenicity of C.Pseudoreteaudii,the function and molecular mechanism of important genes related to disease resistance of Eucalyptus.Finally,it would provide technical support for the prevention and control of Eucalyptus Cyliudrocladium leaf blight.