新型氨基酸制剂对创伤大鼠自由基代谢及免疫功能的影响

Effects of a new crystalline amino acids solution on free radical metabolism and immune function in traumatized rats

目的 :观察富含谷氨酰胺 (Gln)和支链氨基酸 (BCAA)的新型氨基酸制剂对创伤后大鼠自由基代谢以及免疫功能的影响。 方法 :2 1只雄性Wistar大鼠行创伤手术后 ,随机分成对照组、17AA组及 2 0AA组 ,分别饲以酪蛋白、17AA及 2 0AA为氮源的合成饲料 ,各组饲料等氮、等能量 ,实验期 14天。观察实验大鼠术前、术后 3、7、14天体重、摄食量、血浆MDA、SOD活性等指标的动态变化 ,并于实验期结束时测定创伤大鼠伤口胶原蛋白、脾淋巴细胞转化反应等指标。 结果 :①创伤后大鼠体重下降明显 ,血浆MDA含量明显升高而SOD活性显著降低。②与17AA组比较 ,2 0AA组大鼠伤口胶原蛋白合成量增加。③ 2 0AA降低创伤大鼠血浆MDA含量、升高抗氧化酶活性的效用明显好于 17AA。④与 17AA组比较 ,2 0AA组大鼠胸腺、脾重量及脾淋巴细胞转化反应均有增加的趋势。 结论 :新型氨基酸制剂可提高创伤大鼠的抗氧化能力 ,增强机体免疫功能 ,并促进创伤愈合。

Objectives:In this study,the effects of glutamine(Gln) and branched chain amino acids(BCAA) enriched formulas on free radical metabolism and immunity in traumatized rats were investigated. Methods:After injury,twenty one male Wistar rats were randomly divided into three groups,and fed with rations containing casein,a commercial amino acids(17AA),and a new amino acids formula(20 AA) respectively.The rations were isonitrogenous and isocaloric.Before operation and on days 3,7,14 postoperation,body weight,dietary intake,the concentrations of MDA,the activities of SOD in plasma were measured.At last,the animals were killed,the hydroyproline and spleen lymphocyte blastogenesis were determined. Results:①After injury,body weight of rats were reduced significantly,the concentrations of MDA in plasma were increased,while the SOD activities were decreased. ②Compared with 17AA group, the levels of hydroyproline in sponge were increased in 20AA group.③There were better effects of reducing plasma MDA levels and enhancing plasma antioxidase activities in 20 AA group than 17 AA group.④The weight of thymus and spleen and spleen lymphocyte blastogenesis were more obviously increased in 20AA group than in 17AA group. Conclusions:The new amino acids preparations can increase the antioxidase activities,enhance immunity and promote wound healing.