低频电针对吗啡依赖大鼠免疫功能康复的影响

Effects of low frequency electroacupuncture on the rehabilitation of immune function in morphine-dependent rats

目的 观察低频电针对吗啡依赖大鼠免疫功能康复的影响。方法 采用向大鼠腹腔注射吗啡并以纳洛酮催瘾的方法建立吗啡快速成瘾模型。将 40只健康SD大鼠随机分为正常对照组、模型组、模型电针组、模型自动脱瘾组 ,每组 1 0只。对模型电针组于“关元”、“命门”、双“后三里”施以低频电针治疗。免疫荧光法检测各组大鼠外周血中T细胞亚群含量。结果 模型组大鼠外周血CD4+ 含量为 (2 9.37± 1 .75) % ,CD4+ /CD8+ 比值降低至 1 .1 9± 0 .0 8,与正常组大鼠相比差异均有显著性意义 (P <0 .0 1 )。模型电针组大鼠外周血CD4+ 含量为 (36 .94± 3 .81 ) % ,CD4+ /CD8+ 比值亦升高至 1 .45± 0 .1 2 ,与模型组、模型自动脱瘾组相比 ,差异均有显著性意义 (分别为P <0 .0 1与P <0 .0 5) ;而与正常组相比 ,差异无显著性意义 (P >0 .0 5)。

Objective To observe the effects of low frequency electroacupuncture on the rehabilitation of immune function in morphine dependent rats. Methods Acute morphine dependent rats were established by intraperitoneal injection of morphine and provocating addiction with naloxone. 40 healthy rats were assigned randomly to normal group, model group, electroacupuncture group and auto abstinence group. In the electroacupuncture group, the points of 'Guanyuan'?'Mingmeng' and 'Housanli' were selected and treated with electroacupuncture. Then with immunofluorescence technique(IFT), the content of T lymphocyte subsets in peripheral blood of each group was examined. Results The level of CD4 + and the ratio of CD4 +/CD8 + of model group were 29.37±1.75% and 1.19 ±0.08 respectively and decreased significantly when compared with that of normal group ( P <0.01) . The level of CD4 + and the ratio of CD4 +/CD8 + of electroacupuncture group were 36.94±3.81% and 1.45±0.12 respectively and increased significantly when compared with that of model group and auto abstinence group ( P <0.01 and P <0.05 respectively),but the difference between electroacupuncture group and normal group was not significant( P > 0.05 ). Conclusion Low frequency electroacupuncture can promote the rehabilitation of immune function in morphine dependent rats.