创伤性休克大鼠模型的建立

DEVELOPMENT OF AN EXPERIMENTAL MODEL OF TRAUMATIC SHOCK IN RATS

建立一种既符合临床特征 ,又简便易行的创伤性休克动物模型。用 2 5 0 0g铁轮于 3 0cm高度击中SD大鼠双侧股骨中上段造成粉碎性骨折、软组织损伤及出血 ,引发休克 ,维持 90min后复苏 ,监测生命体征 ,计算 2 4、72h内死亡率。结果显示 ,于 3 0cm高度砸伤大鼠 ,(7 8± 1 6)min后达到休克水平 ,2 4h内死亡率为 2 7 5 % ,72h内死亡率为 5 2 5 %。该模型操作简单 ,易于控制 ,模型相对稳定并有一定的死亡率 。

To establish a standard experimental model of traumatic shock in rats that could not only imitate clinical features,but also be performed easily, multiple injuries involving the leg bones,the soft tissue and hemorrhage in Sprague Daulay rats were made by hitting its legs on both sides with a 2500g pontil falling from 30cm height to induce traumatic shock. Resuscitated the rats after 90 minutes and monitored the vital signs,and then calculated the 24 hour and 72 hour death rates. There were about (7 8±1 6) minutes of the rats from injury to shock. The death rates were 27 5% in 24 hours and 52 5% in 72 hours. This model has been proved to be relatively simple,stable and basically closed to clinical features of traumatic shock, so it is a reliable,acute animal model for experimental study of traumatic shock.