摘要
AIM: To evaluate the spatiotemporal expression pattern of PPARγ in embryonic and early postnatal stages of rat retina.METHODS: Fetal rats were collected at 13-18 d of gestation(GD) from pregnant females and postnatal rats at 1d(P1) and 5d(P5) after birth were also used. We used RT-PCR to detect PPARγ m RNA and immunohistochemical to observe PPARγ protein. And at last, we chose HE staining showed the structural changes of rat retina during development.· RESULTS: RT-PCR analysis showed that PPARγm RNA was expressed as early as GD13 and gradually decreased as maturation continued. However, the PPARγgene expression significantly increased after birth,especially in P5. Immunohistochemical analysis showed PPARγ protein was expressed throughout the retinal neuroepithelium at GD13 and GD14, and then decreased during late embryogenesis but remained relatively high in the predicted ganglion cell zone. During postnatal development, PPARγ protein was remarkably increased and the positive signals were mainly located in nerve fiber layer(NFL), ganglion cell layer(GCL) and outer layers of the retina.CONCLUSION: The spatiotemporal changes of PPARγexpression demonstrated that PPARγ might play a role in regulating the differentiation and maturation of retinal cells.
AIM: To evaluate the spatiotemporal expression pattern of PPARγ in embryonic and early postnatal stages of rat retina.METHODS: Fetal rats were collected at 13-18 d of gestation(GD) from pregnant females and postnatal rats at 1d(P1) and 5d(P5) after birth were also used. We used RT-PCR to detect PPARγ m RNA and immunohistochemical to observe PPARγ protein. And at last, we chose HE staining showed the structural changes of rat retina during development.· RESULTS: RT-PCR analysis showed that PPARγm RNA was expressed as early as GD13 and gradually decreased as maturation continued. However, the PPARγgene expression significantly increased after birth,especially in P5. Immunohistochemical analysis showed PPARγ protein was expressed throughout the retinal neuroepithelium at GD13 and GD14, and then decreased during late embryogenesis but remained relatively high in the predicted ganglion cell zone. During postnatal development, PPARγ protein was remarkably increased and the positive signals were mainly located in nerve fiber layer(NFL), ganglion cell layer(GCL) and outer layers of the retina.CONCLUSION: The spatiotemporal changes of PPARγexpression demonstrated that PPARγ might play a role in regulating the differentiation and maturation of retinal cells.
基金
Supported by Science and Technology Project of Nantong,China(No.BK2012070)
