HPLC-FLD和GC-MS测定环境水体中苯并(a)芘的方法比较

Comparison of HPLC-FLD with GC-MS in estimation of Benzo[a] pyrene in environmental water

优化了环境水样中苯并(a)芘的固相萃取过程和HPLC分析方法,建立了苯并(a)芘的GC-MS分析方法,将其应用于焦化厂废水样品的苯并(a)芘分析,比较了净化和不净化样品在定量上面的区别。结果表明,两种方法平均回收率均大于70%,相对标准偏差均小于10%,检出限分别为0.0001μg/L和0.0003μg/L,均能满足测定要求,测定低浓度苯并(a)芘样品时,高效液相色谱法更稳定,峰形基线平稳,检出限低,回收率高。净化和未净化的苯并(a)芘在用GC-MS和HPLC-FLD两种方法定量计算浓度时,无显著性差异。在分析基质复杂样品,为了结果更为准确,保护色谱柱,需要净化。

benzo[a]pyrene( Ba P) in wastewater of a coking plant was determined by HPLC with optimized solid-phase extraction and GC-MS. Of the two methods,the average recovery rates were above 70%,and relative standard deviations were below 10%,and the limits of detection were0. 0001μg / L and 0. 0003μg / L respectively for HPLC and GC-MS,which could satisfy the measurement requirements. When the sample of low Ba P concentration was determined,the result from HPLC method was more stable,with smooth baseline of peaks,low limit of detection and high recovery rate. For purified and raw Ba P samples,the detection results from the two methods showed no significant difference. In order to protect the chromatographic columns and make the analysis more accurate,the purification was needed for complex matrix samples.