二氢杨梅素的分离纯化及其细胞内抗氧化和体外结合胆酸盐能力的研究

Separation, Purification, Cellular Antioxidant Activity, and In Vitro Bile Salt-Binding Ability of Dihydromyricetin

本研究通过水提重结晶法从藤茶中分离纯化得到二氢杨梅素样品,利用电子扫描电镜观察其结晶形态,并采用细胞内抗氧化能力评价法(cellular antioxidant activity assay,CAA)对二氢杨梅素的细胞内抗氧化能力(CAA)进行研究,同时在体外模拟人体胃肠消化环境,测定了二氢杨梅素体外结合胆酸盐的能力。结果表明:利用多次重结晶法对二氢杨梅素进行分离纯化,可得到高纯度的二氢杨梅素样品,得率为43%。细胞内抗氧化试验中二氢杨梅素的EC50值为6.17±0.08μmol/L,CAA值为74.90±0.90μmol QE/100μmol,表明其具有很强的细胞内抗氧化能力。二氢杨梅素对胆酸钠(SC),甘氨胆酸钠(SGC),牛磺胆酸钠(STC)都有较强的结合作用,结合量分别为1.24±0.02、0.97±0.06、1.06±0.002μmol/m L,由结果可知,二氢杨霉素对游离胆酸钠的结合能力最强。

Dihydromyricetin(DMY) was separated and purified from Ampelopsis using water extraction and recrystallization. The crystal morphology was analyzed by scanning electron microscopy and the cellular antioxidant activity(CAA) was determined by CAA assay. Meanwhile, in vitro bile salt-binding ability of DMY was determined by in vitro simulation of the human gastrointestinal environment. The results showed that separation and purification techniques with multiple recrystallizations produced high-purity DMY, and the yield was 43%. An EC50 of 6.17 ± 0.08 μmol/L and CAA of 74.90 ± 0.90 μmol QE/100 μmol were obtained, which indicated that DMY exhibited strong CAA. DMY also exhibited relatively strong binding to sodium cholate, sodium glycocholate, and sodium taurocholate, with binding capacity of 1.24 ± 0.02, 0.97 ± 0.06, and 1.06 ± 0.002 μmol/m1, respectively, with strongest binding capacity for free sodium cholate.