硒酸精氨酸对富硒平菇子实体品质的影响

Effect of Selenoarginine on the Quality of Selenium-enriched Pleurotus ostreatus Fruiting Body

通过向栽培料中添加硒酸精氨酸培育富硒平菇,研究硒酸精氨酸对平菇子实体富硒能力、谷胱甘肽过氧化物酶活性、挥发性物质组成、蛋白含量和蛋白分布等的影响。研究结果表明:平菇子实体中的硒含量与栽培料中的硒浓度线性正相关(R=0.9967),同一栽培料中子实体硒分布为:菌盖>菌柄;硒在多糖中的含量较低,在蛋白质中大量积累,但富硒平菇子实体的蛋白含量和谷胱甘肽过氧化物酶活性没有显著变化;气相色谱结合质谱(GC-MS)分析表明,富硒平菇子实体与普通平菇子实体的挥发性物质组成简单,都以醇类为主,但是富硒平菇子实体中1-辛烯-3-醇相对含量较高,蘑菇风味更加浓郁;十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析表明,平菇子实体中蛋白质分布广泛,但硒对蛋白质的合成代谢途径没有影响,不改变平菇子实体蛋白的分布。

Selenoarginine was added to the cultivation material to produce selenium-enriched Pleurotus ostreatus and the effects on the quality of its fruiting body were investigated, in terms of selenium-accumulation ability, glutathione peroxidase(GSH-PX) activity, volatile compound content, protein content, and protein distribution. The results showed that selenium concentration in the fruiting bodies had a significantly positive correlation(R=0.9967) with that in the cultivation material. Within the same cultivation material, selenium concentration in the cap was higher than that in the fruiting body. A larger amount of selenium was accumulated in proteins than in the polysaccharides. However, there were no significant changes in protein content and glutathione peroxidase activity. Gas chromatography-mass spectrometry(GC-MS) analysis indicated that selenium-enriched Pleurotus ostreatus had a simple composition of volatile compounds, with a relatively high alcohol content, than that of the non-enriched Pleurotus ostreatus. However, the relative content of 1-octen-3-ol in selenium-enriched Pleurotus ostreatus was higher than that of the non-enriched Pleurotus ostreatus. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis indicated that selenium did not change the distribution of proteins in the fruiting bodies of Pleurotus ostreatus.