林蛙油的雌激素样作用研究

Estrogen-like Effects of Oviductus Ranae

本文探讨了林蛙油对生长、激素水平及性腺激素受体表达的影响。采用雌性昆明小鼠24只,随机分为高剂量组、低剂量组和空白组。给药组灌胃林蛙油,空白组灌胃生理盐水。给药结束后,取各组小鼠的卵巢、子宫及肾上腺称湿重,并对其组织切片进行了病理形态学观察,取血清测激素水平。利用Western Blot方法检测小鼠子宫、卵巢及肾上腺蛋白组织中FSHR、TβRⅠ及TβRⅡ的表达。林蛙油对雌性小鼠体重、子宫湿重、卵巢湿重及肾上腺湿重均无显著影响,血清内E2及P的含量显著增加(p<0.05),FSH的含量显著增加(p<0.01),T的含量显著增加(p<0.001),PRL和LH的含量变化无显著差异。林蛙油高剂量给药组肾上腺蛋白组织FSHR的表达水平升高(p<0.05),其他组变化不显著。表明长时间大剂量服用林蛙油,在一定程度上会引起机体内的E2、T、FSH和P激素水平的变化及FSHR蛋白表达的增高,具有一定的雌激素样作用。

The effects of long-term, high-dose oviductus ranae on growth, sexual organs, and hormones levels of mice were investigated. A total of 24, female Kunming mice were randomly assigned to the high-dose, low-dose, and control groups. Test groups received intragastric administration of oviductus ranae, and the control received intragastric administration of saline solution. After treatment, wet weight of the ovaries, uterus, and adrenal glands were measured and processed for histopathology. Hormone levels in the serum were measured by chemiluminescent immunoassay(CLIA), whereas protein expression of follicle-stimulating hormone receptor(FSHR), Type Ⅰ Transform growth factor β receptor TβRI), and TβR II in the uterus, ovary, and adrenal tissue were measured using the western blot. Oviductus ranae did not have a significant effect on the weight of female mice or wet weights of the uterus, ovary, and adrenal glands; while serum E2 and P(p < 0.05), FSH(p < 0.01), and T(p < 0.001) content increased significantly. Oviductus ranae did not have a significant effect on prolactin(PRL) and luteinizing hormone(LH) content. High doses of oviductus ranae led to an increase in the adrenal tissue protein, FSHR(p < 0.05), but did not show a significant effect in other groups. The results show that long-term, high-dose oviductus ranae alters serum E2, T, FSH, and P as well as increases FSHR protein expression. Thus, oviductus ranae exhibits estrogen-like effects, which may lead to the occurrence of reproductive tumors.