卵转铁蛋白对树突状细胞成熟的影响

Effects of Ovotransferrin on the Maturation of Mouse Bone Marrow-derived Dendritic Cells

为考察卵转铁蛋白(OVT)对髓源性树突状细胞(BMDCs)成熟的影响。采用不同浓度OVT单独刺激和脂多糖(LPS)+不同浓度OVT共刺激第6 d小鼠BMDCs 48 h,通过倒置显微镜观察细胞形态,并用流式细胞仪测定各组CD11c+细胞比例以及DCs表面MHC-II,CD80和CD40分子表达量。结果表明,不同浓度OVT组刺激的DCs周围毛刺状突起增加,呈现典型的树突状细胞形态,且上调CD11c+细胞比例以及DCs表面MHC-II、CD80和CD40分子表达量。LPS+不同浓度OVT组中,低浓度(≤25μg/m L)组可提高CD11c+细胞比例,上调MHC-II、CD80和CD40表达量;高浓度(>25μg/m L)组则能下调CD11c+细胞比例和DCs表面MHC-II、CD80和CD40表达量。因此,不同浓度OVT均能刺激DCs的成熟;LPS+不同浓度OVT共刺激DCs,能显著提高DCs的成熟度,但呈现剂量依赖型,即低浓度时,两者表现出协同作用,高浓度时两者表现出拮抗作用。

To explore the effect of ovotransferrin(OVT) on the maturation of mouse bone marrow-derived dendritic cells(BMDCs) in vitro, different concentrations of OVT as well as lipopolysaccharides(LPS) + different concentrations of OVT were used to stimulate a 6-day culture of BMDCs for 48 hours. Changes in DC morphology were observed using inverted phase microscopy, whereas the proportion of CD11c+ cells and expression of MHC-II, CD80, and CD40 molecules were determined by flow cytometry(FC). The results showed that different concentrations of OVT caused an increase in finer dendritic protrusions with the typical morphology of DCs compared to the negative control. The proportion of CD11c+ cells and expression of MHC-II, CD80, and CD40 molecules were all up-regulated as well. Low concentration(≤ 25 μg/m L) of OVT+LPS up-regulated the proportion of CD11c+ cells and the expression of MHC-II, CD80, and CD40, while high concentration(> 25 μg/m L) of OVT+LPS inhibited both, the proportion of CD11c+ cells and the expression of MHC-II, CD80, and CD40. In conclusion, different concentrations of OVT induced the maturation of DCs. LPS + low concentration of OVT induced a synergistic effect, while LPS + high concentration of OVT showed an antagonistic effect.