温度和盐碱度对液体培养发菜生长和生理特性的影响

Effects of Temperature, Salinity, and Alkalinity in Liquid Culture on Growth and Physiological Characteristics of Nostoc flagelliforme

比较不同温度和不同浓度盐碱处理对液体培养发菜(Nostoc flagelliforme)细胞生长和生理特性的影响。80μmol/(m2·s)光照下,采用含有不同浓度的Na Cl和Na2CO3的BG-11培养液,分别在25、35和45℃温度下培养发菜细胞,在培养8、12、16和24h时,分别测定发菜细胞生物量、质膜透性、超氧化物歧化酶活性、丙二醛、脯氨酸、可溶性蛋白以及可溶性糖含量。结果表明,在高温和盐碱作用下,发菜细胞相对外渗率增加,最高达193.2%;丙二醛含量先上升后下降;随着处理程度的提高,发菜细胞超氧化物歧化酶活性和脯氨酸含量先上升后下降;可溶性蛋白含量呈上升趋势,与处理程度呈正相关;可溶性糖含量先上升后下降;与对照相比,随着温度和盐碱浓度的提高,发菜细胞生长速率明显下降。研究表明在不同培养温度下,应用含有不同浓度盐碱的液体培养基人工培养发菜的过程中,发菜可溶性蛋白和可溶性糖具有重要的生理作用,发菜细胞对不同温度和盐碱浓度产生生理的应激响应。

The temperature, salinity, and alkalinity of Nostoc flagelliforme liquid culture were varied and the effects on the growth and physiological characteristics of the cells were studied. Modified BG-11 culture medium containing different concentrations of Na Cl and Na2CO3 was used to culture N. flagelliforme cells at 25 ℃, 35 ℃, and 45 ℃, under light intensity of 80 μmol/(m2·s). Cell biomass of N. flagelliforme, plasmalemma permeability, and superoxide dismutase(SOD) activity, as well as the content of malondialdehyde(MDA), proline, soluble proteins, and soluble polysaccharides were determined at 8, 12, 16, and 24 h of incubation. The results indicated that, under high temperature and saline-alkali stress, the relative plasmalemma permeability of N. flagelliforme cells was increased by 193.2% and MDA content declined after an initial increase. With increase in the duration of treatment, SOD activity and proline content increased at first and then decreased. Soluble protein showed an upward trend, which was positively correlated with the duration of treatment. The soluble polysaccharide content declined after an initial increase. Compared with the growth rate of the control group, that of N. flagelliforme cells decreased significantly with theincrease in temperature and salinity-alkalinity. The results indicate that, during the process of artificial culture of N. flagelliforme in liquid medium with different levels of salinity and alkalinity at different incubation temperatures, soluble proteins and polysaccharides exert an important physiological effect. Thus, different temperatures and salinity-alkalinity can induce physiological response of N. flagelliforme.