基于高通量测序的浓香型白酒窖池古菌群落结构分析

High-throughput Sequencing of the Archaeal Community in Cellar Pit Mud used in Luzhou Flavor Liquor Production

本试验利用罗氏GS junior高通量测序平台分析了30年窖龄的浓香型白酒窖池窖泥古菌群落结构,结果发现:3口窖池样品共获得8706条有效序列,282个OTUs分类;样品中古菌的Shannon曲线值在4~5之间就趋于稳定,Chao曲线的值随着序列数的增加而增长。窖泥中古菌类群主要集中在Euryarchaeota(广古菌门)下的Thermoplasmata class(热原体纲)中的Thermoplasmataceae(热原体科),Methanobacteria class(甲烷杆菌纲)中的Methanobacteriaceae(甲烷杆菌科),Methanococci class(甲烷球菌纲)中的Methanomicrobiaceae(甲烷微菌科)、Methanocorpusculaceae(甲烷八叠球菌科)和Methanosarcinaceae(甲烷粒菌科);Saccharococcus sp.和WCHD3-02也有检测到。其中Thermoplasmataceae和Methanobacteriaceae占绝对优势,分别为39%和27%,其它菌类Methanomicrobiaceae占9%、Methanosarcinaceae占7%和Methanocorpusculaceae占4%、Saccharococcus sp.(糖球菌属)占1%和WCHD3-02占1%,unclassified序列占12%;对甲烷菌类进行深入分析,发现了Methanoculleus marisnigri strain、Methanoculleus bourgensis strain、Methanosarcina siciliae strain、Methanobacterium、Methanocorpusculum和Methanomicrobia等种属的菌种。

The structure of the archaeal community in pit mud from 30-year-old cellars that is used for the production of Luzhou flavor liquor was analyzed by a Roche GS junior high-throughput sequencing platform. A total of 8,706 valid sequences and 282 operational taxonomic units(OTUs) were obtained from samples inthree cellars. The Shannon curves of archaea were stable in the range from 4 to 5 and the value of Chao curves increased with increasing sequence number. The predominant archaeal groups were of the phylum Euryarchaeota: Thermoplasmataceae of class Thermoplasmata, Methanobacteriaceae of class Methanobacteria, as well as Methanomicrobiaceae, Methanocorpusculaceae, and Methanosarcinaceae of class Methanococci. Saccharococcus sp. and WCHD3-02 were also detected. Among these, the dominant archaea groups were Thermoplasmataceae and Methanobacteriaceae, accounting for 39% and 27%, respectively; Methanomicrobiaceae, Methanosarcinaceae, Methanocorpusculaceae, Saccharococcus sp., WCHD3-02, and unclassified sequences accounted for 9%, 7%, 4%, 1%, 1%, and 12%, respectively. Further study on methanogens showed the presence of Methanoculleus marisnigri, Methanoculleus bourgensis, Methanosarcina siciliae, Methanobacterium, Methanocorpusculum and Methanomicrobia.