小麦阿拉伯木聚糖对S180荷瘤小鼠免疫活性及肠道代谢产物的影响

Effect of Wheat Arabinoxylan on the Immunological Activity and Intestinal Metabolites of S180 Tumor-bearing Mice

本文研究了碱提酸水解阿拉伯木聚糖(AX1)和水提酸水解阿拉伯木聚糖(AX2)对S180荷瘤小鼠机体免疫水平及肠道代谢产物的影响。成分分析表明AX1的蛋白质(1.50%)与阿魏酸含量(7.6μg/g AX1)均低于AX2(蛋白质7.97%,阿魏酸366.3μg/g AX2)。二者的重均分子量(Mw)较为接近,约3×104 u。与阴性对照组相比,AX样品可以抑制小鼠肿瘤的增长,特别是AX2,其抗肿瘤能力与灵芝多糖组(GLP)组相当;AX1、AX2均可显著提高小鼠NK细胞的活性,且AX2的作用效果比AX1好;经AX1与AX2灌胃给药的小鼠腹腔巨噬细胞吞噬作用均有所增强,其中AX2组作用效果与GLP相当;此外,与阴性对照组相比,GLP、AX1、AX2对DTH反应具有明显效果(P<0.05)。采用气相色谱法(GC)分析小鼠结肠内容物中短链脂肪酸含量,结果显示AX1和AX2组的短链脂肪酸显著高于阴性对照组和灵芝多糖组(P<0.05)。

Acid-hydrolyzed arabinoxylans extracted with alkali(AX1) and water(AX2) were prepared to study their effects on the immune activity and intestinal metabolites in S180 tumor-bearing mice. Chemical analysis showed that both the protein content(1.50%) and ferulic acid content(7.6 μg/g) of AX1 were lower than those of AX2(protein, 7.97%; ferulic acid, 366.3 μg/g). The weight-average molecular weights(Mw) of AX1 and AX2 were similar and were about 3 × 104 u. Compared with the negative control group, AX samples could inhibit the tumor growth in the mice, especially AX2, whose anti-tumor capability was comparable with that of ganoderma lucidum polysaccharides(GLP). Both AX1 and AX2 could significantly increase NK cell activity in mice; AX2 had a stronger effect than AX1. Besides, AX1 and AX2 could increase the phagocytosis of intraperitoneal macrophage following intragastric administration and AX2 showed a comparable effect with that of GLP. In addition, GLP, AX1, and AX2 had significant effects on the delayed type hypersensitivity(DTH) reaction(P < 0.05) compared with the negative control group. Short-chain fatty acids(SCFA) in colon contents of mice were also detected by gas chromatography and the results showed that SCFA of the AX1 and the AX2 groups were significantly higher than those of the negative control group and the GLP group(P < 0.05).